IL-6 / Interleukin-6 Test

Overview

Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective.

Our Individual Access Peel Heat Seal is a laminate seal compatible with polypropylene plates, featuring 96 individual foil seal spots or 12 strips of individual spots on a removable backing.

These seals result in individually sealed tubes/strips, and they can be removed from polypropylene plates by peeling, even with a plate which has been removed directly from -80°C storage.

Individual Access Peel Heat Seal forms a complete seal to a plate enabling very low temperature uses, including very low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid).

The seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature.

This seal is available as sheets, for use with manual and semi-automated sealers, such as our Semi-Automated Sheet Heat Sealer (using the 59-2005 Individual Access adapter).

Peelable heat sealing foil, 96 individual seals with tabs, or 12 strips each covering 8 wells; suitable for very low-temperature storage/high temperature uses/PCR

Overview

• Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
• No hazardous chemicals required (e.g. phenol or chloroform)
• Effective lysis with Proteinase K and optional lysozyme treatment
• Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
• Wide compatibility with a variety of food products for GMO-DNA isolation
• Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)

This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.

This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping.  

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice)	200 mg 1 mL to 1.5 mL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Samples Tested by PCR

Food Materials	Samples that Have Been Tested by PCR
Plant related	Cereal, Jam, Chocolate, Spices, Sauce
Animal related	Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage)
Dairy product	Milk, Yogurt, Cheese
Pathogens (enriched from food samples)	E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk

Component	Cat. 54500 (50 preps)
Lysis Buffer L	60 mL
Binding Buffer I	7 mL
Buffer SK	30 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Proteinase K	1 vial
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1