Influenza type A M1

• Screening of Algae Toxins Microcystins and Nodularins
• Format: 10 tests (5 tests/5 control)
• Not provided: Water Sample Bottles
• Run Time: 10 Minutes

Description

Attogene’s Microcystin Test Kit (Rapid – Lab) can be used to detect microcystins in liquid samples.

• • Format: 10 tests
• • Run Time: 10 Minutes

The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.

A tiered notification system which takes different actions based on different numeric thresholds for microcytin-LR concentrations in recreational waters has been developed. This is guidance that allows states to take various actions—such as posting information about harmful algal blooms (HABs), issuing a recreational public health advisory, or temporarily closing recreational waters through a no contact advisory—depending on the severity of the bloom event.

Microcystin-LR

Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L

Harmful Algae Bloom – NOAA

Screening of Algae Toxins Microcystins and Nodularins
Format: 10 tests (5 tests/5 control)
Not provided: Water Sample Bottles
Run Time: 10 Minutes

Introduction

Intended Use

For the enumeration of enterococci in water and other liquids by the membrane filtration technique.

Principle and Interpretation

The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.

Formulation

Ingredients	/liter
Tryptose	20.0g
Yeast extract	5.0g
Glucose	2.0g
Disodium hydrogen phosphate	4.0g
Sodium azide	0.4g
2,3,5-Triphenyl Tetrazolium chloride	0.1g
Agar	10.0g
pH 7.2±0.1 at 25°C

Preparation

Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.

Quality Control

Cultural characteristics observed after incubation at 34-38°C for 40-48hours

Quality control strains	Growth	Colony color
Enterococcus faecalis ATCC29212	Good growth,PR≥0.5	deep red coloured colonies
Escherichia coli ATCC25922	Total inhibition	–

Sorage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……

DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-NHCO-PEG4-amine is a carboxyl-reactive building block with extended PEG spacer arm. The hydrophilic PEG spacer arm improves water solubility. In the presence of of activators (e.g. EDC, or HATU), this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.