To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases. For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD+. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.
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To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases. For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD+. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.
IsoPol® SD+ is an engineered version of IsoPol® DNA Polymerase with even stronger strand displacement and higher salt tolerance.
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Quality Control
ArcticZymes is dedicated to the quality of our products. IsoPol® polymerases are is manufactured at our ISO 13485 certified facility in Norway.
Other Products
10 kb DNA Ladder in Ready-to-load format
Product Info
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Product Info
• For sizing and quantification of double strand DNA fragments. • Composed of 5 bands from 10 kb to 48.5 kb. • Premixed with 6X DNA loading buffer for direct gel loading.
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• For sizing and quantification of double strand DNA fragments. • Composed of 5 bands from 10 kb to 48.5 kb. • Premixed with 6X DNA loading buffer for direct gel loading.
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
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Specifications
Features
Specifications
Main Functions
Isolation total RNA from <1g simple plant sample without chloroform
Applications
RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method
Midi spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
Plant leaves, roots, stems, fruits, fungi, etc.
Sample amount
0.5-1 g
Elution volume
≥400μl
Time per run
≤40 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principle
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – medium isolation of several samples can be completed in 40 minutes
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Kit Contents
Contents
R415202
D415203
Purification Times
20 Preps
100 Preps
HiPure DNA Midi Column II
20
100
HiPure RNA Midi Columns II
20
100
15ml Collection Tubes
40
200
Buffer RLC
120 ml
550 ml
Buffer PRC1
120 ml
550 ml
Buffer RW1
70 ml
400 ml
Buffer RW2*
20 ml
100 ml
RNase Free Water
30 ml
120 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Purchase Guide
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Select the right purification kit to get impactful results：
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
endo-BCN-PEG3-Gly represents an ADC linker featuring a BCN group, a hydrophilic PEG3 linker, and glycine residue. The BCN group is a click chemistry handle that readily reacts with azide groups on a target molecule to form stable triazole linkages. The PEG units enhance the aqueous solubility of the compound and may assist in fine-tuning DMPK properties. This compound may be applied to the synthesis of antibody-drug conjugates.
Document
endo-BCN-PEG3-Gly represents an ADC linker featuring a BCN group, a hydrophilic PEG3 linker, and glycine residue. The BCN group is a click chemistry handle that readily reacts with azide groups on a target molecule to form stable triazole linkages. The PEG units enhance the aqueous solubility of the compound and may assist in fine-tuning DMPK properties. This compound may be applied to the synthesis of antibody-drug conjugates.
