Thin polyester heat sealing film which is easily pierceable with autosampler needles/ABI® 3730. The seal is suitable for PCR, qPCR and optical applications.
Detail
Overview
Thin polyester heat sealing film which is easily pierceable with autosampler needles/ABI® 3730. The seal is suitable for PCR, qPCR and optical applications.
Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
Our ClearASeal Pierce heat sealing film is an optically clear polyester backed film, forming a pierceable seal to polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
Its pierceability renders it useful for automation and for use on needle, capillary and tip based liquid handling systems
It is effective on the ABI®3730 capillary sequencer, removing the need for the use of expensive septa mats
The ClearASeal Pierce forms a complete seal to a plate enabling moderately low and high temperature uses, including PCR when using a pressurized heated lid thermal cycler
It demonstrates a moderate solvent resistance and can be utilized for short term compound storage
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
Fasciolosis is one of the most frequently encountered autochthonous helminthic infections in Central Europe. The immunodiagnosis of Fasciola hepatica infections is challenged by high serological cross-reacitivity with other (hepatic) parasitological infections encountered in Central Europe, such as alveolar echinococcosis, toxocarosis and ascariosis, but also other parasitic infections acquired during overseas travel. The SAP-2 recombinant antigen shows a high specificity, especially with patients with other parasitic infections. It is a suitable serological test for routine diagnosis of human fasciolosis, particularly if the results are supported by clinical history.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
77% sensitivity, 98% specificity
Test Principle
The kit provides all the material needed to perform 96 enzyme-linked immunosorbant assays (ELISA) on breakable microtitration wells sensitized with Fasciola hepaticarecombinant antigen. Specific antibodies in the sample will bind to the antigen and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Fasciola hepatica specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.The test can be performed with automatic systems, but this must be validated by the user.
12 x 8 strips (96 tests) Fasciolosis is one of the most frequently encountered autochthonous helminthic infections in Central Europe. The immunodiagnosis of Fasciola hepatica infections is challenged by high serological cross-reacitivity with other (hepatic) parasitological infections encountered in Central Europe, such as alveolar echinococcosis, toxocarosis and ascariosis, but also other parasitic infections acquired during overseas travel. The SAP-2 recombinant antigen shows a high specificity, especially with patients with other parasitic infections. It is a suitable serological test for routine diagnosis of human fasciolosis, particularly if the results are supported by clinical history.
