IST-106 PierceASeal FoilTM Heat Sealing Film

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Purification and enrichment of intact urinary exosomes for functional studies.
• Bind and elute all RNA irrespective of size or GC content, without bias.
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• No phenol extractions, Proteinase K treatment, nor carrier RNA required.
• No time-consuming ultracentrifugation, filtration nor special syringes are required.
• No precipitation reagents nor overnight incubation required.
• Compatible with urine from any species.
• Pure exosomes are purified and are free-from any other RNA-binding proteins.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.

Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of Exosomes Purified	40 nm – 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Overview

• Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrations
• DNA is accurately quantified by using a standard curve constructed from the provided DNA Standards
• Specially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights

Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations.  The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.

Details

Supporting Data

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Storage Conditions
Upon receipt, store Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 61600 (100 reactions)
2X Real-Time PCR Master Mix	1 mL
NGS Library Quantification Primer Set Mix	600 µL
Quantified NGS Library Standards (for Small-RNA Seq)*	5 x 80 µL Standards
Product Insert	1

* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference bands at 500 bp and 1000 bp
• 2686 bp (pUC19) reference band for easy clone identification

The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

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CloneSizer 100 bp DNA Ladder (Cat# 11600) – 100 loads

Ladder Properties:

• Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference bands at 500 bp and 1000 bp
• 2686 bp (pUC19) reference band for easy clone identification

Fragment	Size (bp)	Mass (ng)
1	2686	72
2	2000	53
3	1500	41
4	1200	42
5	1000	56
6	900	30
7	800	29
8	700	25
9	600	25
10	500	52
11	400	19
12	300	20
13	200	19
14	100	17

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.