Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.
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Overview
Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.
Heat sealing is a quick and cost effective method of plate sealing
Our Gas PermASeal II Heat Seal is a paper with a grid lacquer coating which is porous and gas permeable
The Gas PermeASeal II Heat Seal is compatible with polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
The seal can be removed by peeling, or it can be pierced with a pipette tip manually or using a liquid handling robot
The Seal has a temperature range from -20°C to 80°C
It can be used for insect and seed storage, as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in roll format compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
EXTRAClean Urine Exosome Purification and RNA Isolation Kits
Product Info
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Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact urinary exosomes for functional studies.
Bind and elute all RNA irrespective of size or GC content, without bias.
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
No phenol extractions, Proteinase K treatment, nor carrier RNA required.
No time-consuming ultracentrifugation, filtration nor special syringes are required.
No precipitation reagents nor overnight incubation required.
Compatible with urine from any species.
Pure exosomes are purified and are free-from any other RNA-binding proteins.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.
Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
