IST-113 Gas PermASeal IITM Heat Sealing Film

Overview

• Clean-up & concentrate total RNA (including miRNA) in minutes
• Clean-up & concentrate RNA from TRIzol^®, TRI Reagent^®, etc
• Clean-up RNA from contaminants including enzymes, primers, nucleotides
• Rapid spin-column protocol, elute in 20 µL
• 96-well format for high throughput processing & Micro-Elute spin column formats for 8 µL are available
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol^® and TRI Reagent^®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

RNA Clean-Up and Concentration Kit (Spin Column)

Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.

RNA Clean-Up and Concentration 96-Well Kit (High Throughput)

Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.

RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute)

Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.

Protocol

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	10 μg
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material	10 μg of RNA
Minimum Elution Volume	8 μL
Time to Complete 10 Purifications	20 minutes
Average Recovery	≥ 90%

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 23600 (50 preps)	Cat. 43200 (100 preps)	Cat. 25100 (192 preps)	Cat. 61000 (50 preps)
Buffer RL	40 mL	2 x 40 mL	2 x 40 mL	40 mL
Wash Solution A	38 mL	2 x 38 mL	2 x 38 mL	38 mL
Elution Solution A	6 mL	2 x 6 mL	2 x 20 mL	6 mL
Column Activation Solution	–	–	–	30 mL
Micro Spin Columns	50	100	–	–
Micro-Elute RNA Spin Columns	–	–	–	50
96-Well Plate	–	–	2	–
Adhesive Tape	–	–	4	–
Collection Tubes	50	100	–	50
96-Well Collection Plate	–	–	2	–
Elution Tubes (1.7 mL)	50	100	–	50
96-Well Elution Plate	–	–	2	–
Product Insert	1	1	1	1

Overview

Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective.

Our Individual Access Peel Heat Seal is a laminate seal compatible with polypropylene plates, featuring 96 individual foil seal spots or 12 strips of individual spots on a removable backing.

These seals result in individually sealed tubes/strips, and they can be removed from polypropylene plates by peeling, even with a plate which has been removed directly from -80°C storage.

Individual Access Peel Heat Seal forms a complete seal to a plate enabling very low temperature uses, including very low temperature storage, and high temperature uses, such as PCR (when used with a pressurized heated lid).

The seal demonstrates moderate solvent resistance and can be utilized for short term compound storage at room temperature.

This seal is available as sheets, for use with manual and semi-automated sealers, such as our Semi-Automated Sheet Heat Sealer (using the 59-2005 Individual Access adapter).

Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective.

Specification

6.5-65 x continuous zoom stereo microscope		XTL-6565A	XTL-6565B	XTL-6565D	XTL-6565C
Zoom Body	Binocular head,45°decline,360°rotate	●		●
Zoom Body	Trinocular head,45°decline,360°rotate		●		●
Eyepiece	WF10X22mm	●	●	●	●
Objective	zoom objective 0.65X—6.5X	●	●	●	●
Zoom Rate	1:10	●	●	●	●
Total Amplific- ation Rate	the standard configuration is 6.5X-65X	●	●	●	●
Auxiliary Lens	0.3X,0.5X,0.7X,1X,1.5X,2X For Option	O	O	O	O
WorkingDistance	110mm	●	●	●	●
Djopte	55mm-75mm	●	●	●	●
Focus arm	76mm Diameter,32mm pole size	●	●	●	●
plate	Black/White plate,Glass plate for option	●	●	●	●
Dptiona	Stand	O	O
Stand	BL3 base,column support upper and lower light source 3 WLED	●	●
Stand	811 base column support large flat base has no light source			●	●
External light source	LED illumination	○	O	O	O
Optional	Photography,camera accessories CCD Coupler	〇	Q	Q	o
Optiona	Display attachment Monitor	O	O	O