Overview

• Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
• Rapid and convenient spin column protocol
• 96-well format available for high throughput
• High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more

This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 10⁹ viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.

Details

Supporting Data

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Kit Specifications (Spin Columns)
Input	2 x 109 bacterial cells
Column Binding Capacity	25 µg
Average Yield*	Up to 20 µg
Time to Complete 10 Purifications	1 hour

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component	Cat. 17900 (50 preps)	Cat. 17950 (192 preps)
Resuspension Solution A	20 mL	60 mL
Lysis Buffer P	18 mL	60 mL
Solution BX	28 mL	110 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Buffer B	30 mL	2 x 30 mL
Proteinase K	12 mg	50 mg
Spin Columns	50	–
96-Well Plate	–	2
Adhesive Tape	–	4
Collection Plate	–	2
Collection Tubes	50	–
Elution Tubes (1.7 mL)	50	–
Elution Plate	–	2
Product Insert	1	1

Introduction

This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.

Details

Kit Contents

Contents	D635901D	D635902D	D635903D
Purification Times	48	96	480
MagPure Particles N	1.1 ml	2.2 ml	11 ml
Buffer ATL	40 ml	80 ml	2 x 200 ml
Buffer BGL	40 ml	80 ml	2 x 200 ml
Buffer BST1	60 ml	120 ml	550 ml
Buffer GW2*	20 ml	25 ml	2 x 50 ml
Proteinase K	48 mg	110 mg	2 x 220 mg
Protease Dissolve Buffer	5 ml	10 ml	30 ml
DTT	235 mg	235 mg	3 x 235 mg
Elution Buffer	10 ml	10 ml	30 ml

Prefilled Kit Contents

32/48 channel machine

Product	Contents and volume	D6359D-TL-06
Buffer BGL		80 ml
Buffer ATL		80 ml
Proteinase K		110 mg
Protease Dissolve Buffer		10 ml
DTT Powder		2 x 235 mg
Elution Buffer		5 ml
AS-Tip		12 pcs
2.0ml V bottom plate	Row 1/7：empty	6 plates
	Row 2/8：500μl Buffer BST1
	Row 3/9：500μl Buffer BST1
	Row 4/10：20μl MagPure Particles N 500μl Buffer GW2 Row 5/11：500μl Buffer GW2 (ethanol ~350ul)
	Row 6/12：60μl Elution Buffer

96 channel machine

Product	Contents and volume	D6359D-F-96	D6359D-F-48
Buffer BGL		80 ml	40 ml
Buffer ATL		80 ml	40 ml
Proteinase K		110 mg	48 mg
Protease Dissolve Buffer		10 ml	5 ml
DTT Powder		235 mg	235 mg
Elution Buffer		5 ml	5 ml
96-Tip (AS)		1	1
Sample Plate 1	500μl Buffer BST1	1	1
Sample Plate 2	500μl Buffer BST1	1	1
Wash Plate 1	20μl MagPure Particles N500μl Buffer GW2	1	1
Wash Plate 2	500μl Buffer GW2	1	1
Elute Plate	60μl Elution Buffer	1	1

Storage and Stability

Proteinase K and DTT Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.

This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.

Introduction

Usages:
For selective cultivation of bacteria especially Vibrio.

Principle:
Peptone provide carbon and nitrogen sources; sodium chloride to maintain osmotic equilibrium; higher pH can suppress the growth of coliform and other bacteria, it is conducive to the growth of Vibrio cholerae.

Formulation（per liter）:
Peptone 10g
Sodium chloride 10g
Final pH 8.5 ± 0.2

How to use：
1.Suspend 18g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Quality control：
          　　

Item	The name and number of strain	Growth	Colony Color
1	Vibrio cholerae VbO	Good	Turbid broth
2	Escherichia coli ATCC25922	Poor	—

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle 

250g