IST-114 PeelASeal Foil SuperTM Heat Sealing Film

Overview

• Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive
• Bead tubes (provided) allow for effective mechanical homogenization
• Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more

These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.

Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)

This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.

Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)

Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.

Details

Supporting Data

Figure 1 / 6

Previous

Next

Click for expanded view

Kit Specifications
Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	All sizes
Maximum Amount of Starting Material: Fungi (Wet weight) Yeast or Gram-positive bacterial culture	50 mg 0.5 mL – 1 mL
Time to Complete 96 Purifications	40 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. 27300 (50 preps)	Cat. 27350 (192 preps)
Lysis Buffer L	30 mL	2 x 60 mL
Resuspension Solution A	20 mL	60 mL
Solution BX	28 mL	2 x 28 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Buffer B	15 mL	30 mL
Bead Tubes	50	200
Spin Columns	50	–
Collection Tubes	50	–
96-Well Plate	–	2
96-Well Collection Plate	–	2
Adhesive Tape	–	4
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1

Introduction

Usage: For isolating lactose-fermenting Gram-negative enteric bacilli.

Approximative Formula(Per Liter)

Pancreatic Digest of Gelatin                    17g

Peptones(meat and casein)                      3.0g

 Lactose Monohydrate                             10g 

Sodium Chloride                                      5g

 Bile Salts                                               1.5g

Agar                                                       13.5g

 Neutral Red                                          30mg 

Crystal Violet                                        1mg

Final pH 7.1±0.2  

Directions: Suspend 50g in 1 L of distilled or deionized water. Heat with frequent agitation to dissolve. Boil 1~2min. Distribute into flasks. Cool to 45~50°C and pour into sterile petri dishes. 

Storage: Keep container tightly closed, store in a dry place, away from bright light.

500g

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

1. Champion™ Competent Cells
2. pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
3. Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 

Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.