IST-124 QuickSeal Gas PermTM Self Adhesive Sealing Film

Features:

Large capacity water capture freeze dryer

Tabletop medium-sized freeze dryer

Suitable for drying medium-sized samples

Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.

product description:

9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity

-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents

Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes

Direct-capture large coil cold trap, higher efficiency, better ice capture effect

Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water

The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents

One-click hot gas defrost, with defrost compressor protection program.

Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease

High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate

The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar

Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier

Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement

Optional configuration of eutectic point detection system and data export system.

The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient

Techinical parameter

9-12L Floor tabletop and floor standing one for lab usage

Tabletop	BD1-9	BD2-9	BD1-12	BD2-12
Floor standing	BH1-9	BH2-9	BH1-12	BH2-12
Water Capture capacity	9L		12L
Cold trap temperature	-70 C	-90℃	-70C°	-90℃
Cold trap volume	15L		20L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional
Vacuum pump	Extreme vacuum 4 * 10-4mbar, pumping capacity ≥ 200L/can be equipped with various types of pumps
Oil mist filter	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16  ISO-KF L-1.5m
anti-corrosive treatment	Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	1-48 ports/optional
Eutectic point judgment	Support/Optional
Data audit trail	Support/Optional
Power	0.9kw	1.5kw	1kw	1.6KW
Size	390*468*448mm	480*498*487mm

Overview

• Reduce endotoxin levels to 0.1 EU/µg DNA or less
• Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
• Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
• Fast and easy processing using a rapid spin-column format

These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.

Endotoxin Removal Kit (Mini)

This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.

Endotoxin Removal Kit (Midi)

This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

Endotoxin Removal Kit (Maxi)

This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Details

Supporting Data

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Kit Specifications
Maximum DNA Input	25 μg
Final Endotoxin Level	≤ 0.1 EU/µg DNA
Size of DNA Purified	Up to 13,000 bp
Maximum DNA Volume Input	100 μL
Average Recovery	> 90%
Time to Complete 10 Purifications	20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 22700 (25 preps)	Cat. 52200 (10 preps)	Cat. 21900 (4 preps)
Buffer SK	15 mL	60 mL	60 mL
Wash Solution H	18 mL	18 mL	18 mL
Elution Buffer I	6 mL	12 mL	12 mL
Endotoxin Removal Solution	1.5 mL	1.5 mL	1.5 mL
Precipitation Solution	–	1.5 mL	1.5 mL
Spin Columns (assembled with Collection Tubes)	–	10	4
Spin Columns	25	–	–
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Elution Tubes (15 mL)	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1	1

Description 

The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. 

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control 

Under suggested conditions, PM5100 ExcelBand™ 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage

The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.