Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 10⁷) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from yeast cultures
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Yeast culture
Sample amount	Bacterial culture: 1-1.5 ml
Elution volume	≥30μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 40 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• High recovery – DNA can be recovered at the level of PG
• Sufficient components – lysozyme, protease K, RNase A and glass beads

Kit Contents

Contents	D314702	D314703
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Glass Beads (0.4~0.6mm)	20 g	90 g
Buffer SE	30 ml	150 ml
Lyticase	1.8 ml	5 x 1.8 ml
Buffer ATL	30 ml	150 ml
ReagentDX	500 μl	1500 μl
Buffer DL	30 ml	150 ml
Buffer GW1*	13 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Proteinase K	12 mg	60 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer AE	15 ml	30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (

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Propargyl-PEG13-t-butyl ester enables Click Chemistry reactions with azide-bearing compounds or biomolecules to form stable triazole linkages; copper is required as a catalyst. The t-butyl protection can be removed under acidic conditions. The PEG units improve the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

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