IST-125 QuickSeal MicroTM Self Adhesive Sealing Film

Propargyl-PEG5-CH2CO2-NHS is an amine reactive linker with an alkyne moeity which can react with azide-bearing biomolecules in copper catalyzed Click Chemistry reactions. The PEG units enhance the solubility of the linker in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-CH2CO2-NHS is an amine reactive linker with an alkyne moeity which can react with azide-bearing biomolecules in copper catalyzed Click Chemistry reactions. The PEG units enhance the solubility of the linker in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Product Description

Isothermal Nucleic Acid Amplification Kit, 39-42℃ & 5-20 Minutes, 14-Month Validity

Product Detail

 Kit Storage and Term of Validity

 Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

 Term of Validity: 14 months 

Isothermal Nucleic Acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit Basic
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Colloidal gold probe:Require a sequence of 46-52nt in length

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Introduction

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.

Details

Specifications

Features	Specifications
Concentration	70 mg/ml
Appearance	Suspension of yellowish brown particles
Surface functional group	Si-OH, Silanol
Dispersibility	Polydisperse Amorphous
Particle size	0.2-2 μm
Preservation conditions	Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth.
Magnetic response speed	~60 seconds
Settling velocity	>10 minutes
High salt mediated binding	>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding	2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding	The recovery of DNA/RNA was up to 85%
DNase/RNase	Not detected
DNA residue	<1 ppm
Recommended application	Plasmid extraction, gel DNA recovery, viral nucleic acid isolation

Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

Ordering information

CAT.No.	Product Name	Package
C14110	MagPure Particles N	100 ml
C14111	MagPure Particles N	400 ml
C14112	MagPure Particles N	3 x 400 ml
C14113	MagPure Particles N	10 x 400 ml

Purchase Guide

Features	MagPure Particles	MagPure Particles N	MagPure Particles G	MagPure Particles F	MagBind Particles
Cat.No.	C1410	C1411	C1412	C1414	C1413
Concentration	100mg/ml	70mg/ml	40mg/ml	50mg/ml	10mg/ml
Form	Amorphous and Porous	Amorphous and Porous	Porous	Amorphous	Nonporous
Surface function	Si-OH, Silica Beads	Si-OH, Silica Beads	Si-OH, Silica Beads	Si-OH, Silica Beads	COOH, Carboxyl Beads
Dispersion	Polydisperse	Polydisperse	Monodisperse	Monodisperse	Monodisperse
Particle Size	1.5-5μm	0.2-2μm	1-1.5μm	0.2-1.5μm	0.8-1μm
Color	Black	Yellowish Brown	Dark Brown	Dark Brown	Yellowish Brown
Magnetic response	15-30s	~60s	~30s	20s	120s
Settling Time (1ml)	>5min	>10min	>3min	>3min	>2h
Usage (0.2ml Sample)	20μl	20μl	20-30μl	20-30μl	20-30μl
DNA Recover Rate (only 4M GITC)	>80%	>80%	>80%	>80%	0
DNA Recover Rate (10% PEG8000/NaCl)	>85%	>85%	>85%	>85%	>90%
Recommended Use	gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification	DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up	Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation	Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction	DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays

• The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.