IST-130 QuickSeal qPCR Crystal UltraTM Self Adhesive Sealing Films
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Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.
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Overview
Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.
Crystal clear seal specifically developed for optical applications, particularly qPCR
It is non sticky when removed from the packaging; this aids handling when wearing gloves
The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
Other Products
Internal Lane Standard (60bp – 600bp, ROX) for ABI Genetic Analyzer
Product Info
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Product Info
Overview
Ready-to-use
Highly stable
Precise
22 discrete bands from 60 bp to 600 bp
High quality ready-to-use double-stranded DNA ladder derived by recombinant technology for precise size and mass determination in various fluorescence-detection instruments including different models of Applied Biosystems® PRISM® and Genetic Analyzers
22 discrete bands, ranging from 60 bp to 600 bp
The ladder is asymmetrically labelled with ROX that can be detected by excitation at 576 nm and fluorescent emission at 597 nm
Compatible with products for fragment analysis including Promega PowerPlex® 16 System
Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 50 µL to 10 mL
Plasma/Serum Exosome Purification Mini Kit (50 µL – 1 mL Plasma/Serum)
Plasma/Serum Exosome Purification Midi Kit (1 mL – 4 mL Plasma/Serum)
Plasma/Serum Exosome Purification Maxi Kit (4 mL – 10 mL Plasma/Serum)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any RNA-binding proteins
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal transcripts.
NanoSight® Analysis
Exosomes enriched with Norgen’s Plasma/Serum Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration
Exosomal RNA Analysis
To purify exosomes and isolate exosomal RNA, choose the Plasma/serum Exosome Purification and RNA isolation kits. The protocol is divided into 2 parts and an aliquot of purified exosomes can be taken for applications like NTA/TEM etc. before processing them for RNA isolation. Or you can use the Exosomal RNA Isolation Kit if you’ve already purified exosomes using a Norgen kit or another method. . Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA. This RNA is ideal for gene expression analysis using RT-qPCR, microarray, or NGS and for biomarker discovery.
Variable depending on the plasma/serum input volume
Time to Complete 10 Purifications
15 – 30 minutes
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Important Note This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
