IST-130 QuickSeal qPCR Crystal UltraTM Self Adhesive Sealing Films
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Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.
Detail
Overview
Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.
Crystal clear seal specifically developed for optical applications, particularly qPCR
It is non sticky when removed from the packaging; this aids handling when wearing gloves
The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
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HCM056 Nutrient Broth
Product Info
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Product Info
Introduction
Usage
For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection.
Formula (per liter)
Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2
How to use
1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples.
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
RNA Basic Isothermal PCR Kit With High Accuracy And Efficiency
Product Info
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Product Info
Product Description
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
RNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Document
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA)
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
Yield
DNA: 1 – 10 μg, RNA: 1 – 25 μg
Principle
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA / DNA can be directly used in various downstream applications
Safe – no phenol chloroform extraction required
Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
Post digestion sorting – higher DNA and RNA yields
High RNA yield – monodisperse carbonyl adsorption
Kit Contents
Contents
R632701
R632702
R632703
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagBind Particles
1.1 ml
2.5 ml
11 ml
MagPure Particles N
1.1 ml
2.5 ml
11 ml
Proteinase K
24 mg
48 mg
220 mg
Protease Dissolve Buffer
3 ml
10 ml
15 ml
Buffer DPS
50 ml
100 ml
2 x 250 ml
Buffer ATL
20 ml
30 ml
120 ml
Buffer BST1
20 ml
40 ml
200 ml
Buffer BXW1*
44 ml
110 ml
3 x 110 ml
RNase Free Water
15 ml
30 ml
120 ml
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
