Standard profile polypropylene, universal 96 well non-skirted plate, designed for use on standard thermal cyclers.
Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.
Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.
The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent |
| Applications | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
| Purification method | Mini spin column |
| Purification technology | Silica technology, acidphenol / guanidine extraction technology (MagZol pretreatment technology) |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Blood, serum, plasma |
| Sample amount | 250μl |
| Elution volume | ≥20μl |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100μg |
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Advantages
Kit Contents
| Contents | R416302 | R416303 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 50 | 2 x 125 |
| MagZol LS Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 60 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.
Product Description
High-Quality Nucleic Acid Reagent for PCR
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Features:
Technical Parameters:
| Product Name | Technical Parameters |
|---|---|
| Nucleic Acid Extraction Reagent | Application: DNA Extract Form: Liquid Shelf Life: 12 Months Brand: Amp-future Bio Automated Nucleic Acid Extractor, DNA Extraction Kit, Automated Nucleic Acid Extractor |
Applications:
Amp-future Bio Nucleic Acid Extraction Reagent is a liquid reagent specially formulated for automated nucleic acid extraction. It is widely used in DNA/RNA extraction from various sample matrixes, such as blood, tissue, cells, bacteria, etc. It ensures high-quality nucleic acid extraction in a short period of time. The reagent is produced in China and has a shelf life of 12 months.
Amp-future Bio Nucleic Acid Extraction Reagent is suitable for use with the Automated Nucleic Acid Extractor. It is highly efficient and reliable, allowing for fast and accurate nucleic acid extraction. The reagent is also suitable for use with the DNA Extraction Kit, which makes it ideal for clinical laboratories. It provides superior nucleic acid extraction performance.
Amp-future Bio Nucleic Acid Extraction Reagent is an essential product for laboratories that need to extract nucleic acids from various sample matrixes. It is highly reliable and efficient, providing superior results in a short period of time. The reagent is also suitable for use with the Automated Nucleic Acid Extractor and the DNA Extraction Kit. It is a reliable and cost-effective product for laboratories that require high quality nucleic acid extraction.
Customization:
Customized Nucleic Acid Reagent Service
Amp-future bio provides custom nucleic acid reagent services to meet your specific needs. We specialize in the production of DNA/RNA extraction reagents for automated nucleic acid extraction, purification and other related applications.
Our products are suitable for DNA extraction, automated nucleic acid extraction, nucleic acid purification, and other related applications. We are confident that our products can meet the highest quality standards and provide the best performance. We are dedicated to providing the best customer service and technical support for our customers.
Support and Services:
Our team of experts provide the highest quality technical support and services for Nucleic Acid Reagents products.
Our technical support staff are available to assist you with any questions or troubleshooting related to the use of Nucleic Acid Reagents. Our team can provide guidance on the proper use and handling of our products, as well as any other technical questions. We offer technical support via phone, email, and online chat.
We also offer a variety of services to support our customers in the use of Nucleic Acid Reagents. These services include:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
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