Introduction

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from insect tissue
Applications	PCR, southern bolt and virus detection, etc
Purification method	96 well DNA plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
• Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
• Safe – without phenol chloroform extraction and alcohol precipitation

Kit Contents

Contents	D313901	D313902
Purification Times	1 x 96	4 x 96
HiPure DNA Plate	1	4
2.2 ml Collection Plate	1	4
1.6 ml Collection Plate	1	4
0.5ml Collection Plate	1	4
Seal Film	8	32
Buffer ITL	30 ml	120 ml
Buffer IL	30 ml	125 ml
Buffer GW1	44 ml	2 x 110 ml
Buffer GW2	50 ml	3 x 50 ml
Proteinase K	50 mg	200 mg
Protease Dissolve Buffer	6 ml	15 ml
Buffer AE	20 ml	60 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

 Features 

1.Brushless frequency motor,in great torque, free maintenance, no powder pollution, quick in speed up and down.  

2.Microprocessor control

3.Digital display in time, speed and RCF.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection. 

6.High quality steel centrifuge body , stainless steel chamber, 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction

 TG16 Technical Parameter：

Max. Speed	16000rpm
Max. RCF	20600×g
Max. Capacity	6×100ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (L x W x Hmm)	513×370×320mm
Net Weight/Gross weight(Kg)	48KG/55kg
Certificates	CE,ISO & Calibration report are available

 Matched Rotor for TG16 

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
G16-1	Angle rotor	14000	4×8PCR	12070
G16-2	Angle rotor	13000	6×8PCR	16080
G16-3	Angle rotor	14000	8×8PCR	13390
G16-4	Angle rotor	13000	12×8PCR	17220
G16-5	Angle Rotor	15000	30×0.5ml	18510
G16-6	Angle Rotor	14000	40×0.5ml	19970
G16-7	Angle Rotor	16000	12×1.5/2ml	17940
G16-8	Angle Rotor	15000	24×1.5/2ml	20600
G16-9	Angle Rotor	13500	30×1.5/2ml	19340
G16-10	Angle Rotor	11000	48×1.5/2ml	12840
G16-11	Angle Rotor	15000	16×5ml	19350
G16-12	Angle Rotor	14000	12×7ml	16370
G16-13	Angle Rotor	14000	6×10ml	16460
G16-14	Angle Rotor	13000	12×10ml	14510
G16-15	Angle Rotor	10000	12×15ml	11840
G16-16	Angle Rotor	5000	24×15ml	3080
G16-17	Angle Rotor	5000	30×15ml	3830
G16-18	Angle Rotor	12000	8×20ml	14510
G16-19	Angle Rotor	12000	6×30ml	14000
G16-20	Angle Rotor	11000	6×50ml	13480
G16-21	Angle Rotor	12000	6×70ml	10810
G16-22.1	Angle Rotor	12000	4x100ml	14850
G16-22	Angle Rotor	8000	6×100ml	7280
G16-23	Angle Rotor	12000	24 pieces capillary vessel	15800
G16-24	Microplate rotor	4000	2×3×48 Well	2300

Propargyl-PEG12-methane is a PEG linker that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The PEG chain enhances the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG12-methane is a PEG linker that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The PEG chain enhances the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.