Propargyl-PEG6-t-butyl ester is a heterobifunctional linker consisting of an alkyne group and a t-butyl protected carboxyl group. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG6-t-butyl ester is a heterobifunctional linker consisting of an alkyne group and a t-butyl protected carboxyl group. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

Usages:
For the growth of pathogenic and non-pathogenic microorganisms.

Principle:
Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.

Formulation (per liter):
Peptone: 10g
Beef extract powder :10g
Sodium chloride: 5g
Agar :15g
Final pH 7.4 ± 0.2

How to use:
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, ,heated to boiling stirring until completely dissolved, dispensing into flask, 121 autoclaved 15min.
2.After the medium is cooled to about 50 , sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare.
3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time.
4. Observe the results.

Quality control:
The following were inoculated after 36 ± 1 for 24h , the results are as follows:
bacteria name bacteria No. growth status colony characteristics
Escherichia coli ATCC25922 not good hemolysis

Staphylococcus aureus, ATCC6538 good β-hemolytic

Beta-hemolytic streptococcus CMCC (B) 32210 good β-hemolytic

Streptococcus pneumoniae CMCC (B) 31001 good α-hemolytic

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

500g

Introduction

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Products	RNA, miRNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Clinical tissues, cells, lymphocytes
Sample amount	Tissue: <20 mgCells: <5 x 106
Yield	2-50μg
Elution volume	≥30μl
Time per run	≤25 minutes

Principle

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.

Advantages

• Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
• High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast – the whole extraction only takes 15-25 minutes
• Nontoxic – no toxic phenol chloroform extraction is required in the extraction

Kit Contents

Contents	IVD4121
Purification Times	50 Preps
HiPure DNA Mini Column Ⅱ	50
HiPure RNA Mini Columns	50
2ml Collection Tubes	150
Proteinase K	24 mg
Protease Dissolve Buffer	1.8 ml
DNase I	600 μl
DNase Buffer	6 ml
Buffer RTL	40 ml
RNA Digestion Buffer	15 ml
Buffer RWC*	20 ml
Buffer RW2*	20 ml
Nuclease Free Water	10 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.