Overview

• High quality DNA, free from RNA contamination
• Isolate genomic DNA from anticoagulated and untreated blood
• Rapid and convenient spin column procedure
• Isolate DNA from inputs as low as 20 µL

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column.  The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes.  The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

Details

Supporting Data

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Kit Specifications
Maximum Blood Input	3 x 3 mm diameter punches
Average yield* (ng)	150 ng
Column Binding Capacity	> 50 µg
Time to Complete 10 Purifications	35 minutes

 * Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 36000 (50 preps)
Digestion Buffer B	6 mL
Lysis Buffer B	20 mL
Wash Solution WN	18 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	1.2 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes	50
Product Insert	1

Propargyl-PEG5-t-butyl ester has one of the functional groups protected by t-butyl group. The protection can be removed under acidic conditions. The propargyl group forms triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The PEG units help improving the water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-t-butyl ester has one of the functional groups protected by t-butyl group. The protection can be removed under acidic conditions. The propargyl group forms triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The PEG units help improving the water-solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1-6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	1-6ml
Elution volume	≥50μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour
• High purity – OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0

Kit Contents

Contents	IVD5435
Purification Times	50
MagPure Particles F	14 ml
Carrier RNA	310 μg
Proteinase K	240 mg
Protease Dissolve Buffer	15 ml
Buffer SDS	15 ml
Buffer MLK	500 ml
Buffer MAW1	250 ml
Buffer MW2*	50 ml
Elution Buffer	60 ml

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.