Grey, low profile PCR plate with a rigid two-component hardshell design which eliminates warping and distortion during PCR testing.
Grey
Polycarbonate frame
Low-profile PCR plate
Rigid two-component design eliminates warping and distortion during PCR
96W Clear Thin Walled PP Tubes
Full Skirt
Packaged 10 plates per sleeve, 50 plates per box
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.
Non-specific amplification is prevented by using this Taq DNA polymerase variant as the DNA polymerase is hotstart-formulated. The 10x reaction buffer supplied together with the DNA polymerase has been specifically designed for optimal PCR performance and DNA polymerase activity. This allows the use of this DNA polymerase in a wide range of PCR applications.
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(endo-BCN-PEG2-ethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The DBCO groups are commonly used for copper-free Click Chemistry reactions due to their strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility.
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(endo-BCN-PEG2-ethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The DBCO groups are commonly used for copper-free Click Chemistry reactions due to their strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility.
Name of Product
Acanthocheilonema viteae – IgG ELISA
Catalog Number
AF 9400
Short Info
This ELISA kit is for the quantitative detection of IgG antibodies against various filiarial nematodes in human serum
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Acanthocheilonema viteae antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
Brief Instructions
Storage
2-8° C
Components
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution
Name of Product
Acanthocheilonema viteae – IgG ELISA
Catalog Number
AF 9400
Short Info
This ELISA kit is for the quantitative detection of IgG antibodies against various filiarial nematodes in human serum
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Specific antibodies in the sample bind to Acanthocheilonema viteae antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
