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IVD3020 MagPure Universal RNA Kit

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This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Detail

Introduction

This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total RNA from tissue, cell
ApplicationsRT-PCR, cDNA synthesis, second generation sequencing
Purification methodPolydisperse magnetic beads
Purification technologyMagnetic beads technology
Process methodManual or automatic
Adaptive instrumentNucleic acid extractor, pipetting workstation
Sample typeTissues, cells, lymphocytes and other clinical sample
Sample amountCells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg


Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water

Advantages

  • High purity – OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy – less than 50% of the price of Qiagen and other imported products
  • Automatic – extraction without manual participation, saving time and effort
  • Universal – suitable for various clinical samples

Kit Contents

ContentsIVD3020
Purification Times200 Preps
MagPure RNA Particles7 ml
DNase I4 x 600 µl
DNase Buffer80 ml
RTL Lysis Buffer150 ml
Buffer MCB*75 ml
Buffer MW1*110 ml
Buffer MW2*50 ml
RNase Free Water60 ml
Cat.NoReagentIVD3020-F-96
DNase Buffer60 ml
DNase I2 x 600 μl
RTL Lysis Buffer80 ml
Buffer MCB18 ml
96-Tip1
Sample plate (DW Plate)500μl Buffer MCB1
Wash 1 Plate (DW Plate)700μl Buffer MW130μl MagPure RNA Partilces1
DNase PlateEmpty
Wash 2 Plate (DW Plate)700μl Buffer MW11
Wash 3 Plate (DW Plate)900μl Buffer MW21
Elution plate (DW Plate)80μl RNase Free Water1
Cat.NoReagentIVD3020-TL-06
Purification times96 Preps
DNase I2 x 600 μl
DNase Buffer60 ml
RTL Lysis Buffer60 ml
Buffer MCB40 ml
96-Tip12 PCS
2.0ml V-bottom plateRow 1/7:500μl Buffer MCBRow 2/8:500μl Buffer MW1Row 3/9:emptyRow 4/10:30μl Magpure RNA Particles500μl Buffer MW2
Row 5/11:900μl Buffer MW2
Row 6/12:80μl RNase Free Water
6

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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Dilution Range1:200

Aluminum Block Set

The Opentrons® 3-piece Aluminum Block Set is used with our Temperature Deck. The set comes with a 24-well, 96-well and flat bottom plate format block. These are used to keep your 2mL tubes, 1.5mL tubes, 96-well PCR plates, PCR strips and flat bottom plates at a constant temperature. These blocks holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).

Stable PCR Isothermal Amplification Kit For Genetic Engineering

Product Description

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

ParametersDetails
Product NameRNA Isothermal Amplification Kit NFO
ManufacturerAmp-future
Storage Temperature-20°C
Kit ComponentsEnzymes, Buffers ,Reagents
Packaging48 Tests/box
Detection Limit500-1000copies/µL
ShippingICE
Test Time5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.