The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Detail
Introduction
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation total RNA and DNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
Advantages
Post digestion sorting – higher DNA and RNA yields
Economy – the price is much lower than imported reagents
Kit Contents
Contents
IVD3026
Purification Times
200 Preps
MagBind Particles
9.0 ml
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer DPS
150 ml
Buffer FRL
40 ml
Buffer ATL
40 ml
Buffer AL
80 ml
Buffer BXW1*
110 ml
RNase Free Water
30 ml
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Introducing the SARS-CoV-2 Detection Kit – the perfect solution for quick and accurate COVID-19 infection testing. This revolutionary kit has been designed to detect the presence of SARS-CoV-2 in Nasopharyngeal swabs, providing you with a reliable and convenient way to determine whether individuals have been infected with the virus. With the ability to perform 48 tests per kit, our detection kit is the perfect solution for rapid and accurate testing.
Our detection kit is designed for maximum accuracy and reliability, with components that must be stored at -20°C to ensure optimal performance. Our convenient shipping process ensures that the kit arrives frozen and ready for use, so you can rest assured that you’ll have the results you need as quickly as possible.
The SARS-CoV-2 Detection Kit is the most reliable and accurate COVID-19 infection testing solution on the market. With its convenient packaging and easy-to-use design, our detection kit is the perfect solution for any medical facility looking to quickly and accurately test for the presence of SARS-CoV-2.
Keywords: Covid-19 Virus Detection Test Kit, Covid-19 Test Kit, Covid 19 Rapid Test Kit
Technical Parameters:
Parameter
Value
Product Name
SARS-CoV-2 DetectionORF1ab Gene NFO Kit
Manufacturer
Amp-future Company
Detection Limit
500-1000copies/µL
Sample Type
Nasopharyngeal Swab
Target Gene
ORF1ab Gene
Packaging
48 Tests/Box
Test Time
5-20mins
Kit Components
Primers, Probes, Enzymes, Buffers
Shipping
ice
Storage Temperature
-20°C
Applications:
Amp-future bio’s SARS-CoV-2 Detection Kit is a rapid test kit for the detection and identification of SARS-CoV-2 virus. This test kit is designed to detect the presence of SARS-CoV-2 virus in human sample materials, and it is suitable for detecting the virus in nasopharyngeal swab samples. It has a detection limit of 500-1000 copies per µL, and it is packaged with 48 tests per kit. The kit uses ORF1ab Genetarget gene for the detection of SARS-CoV-2 virus.
The Amp-future bio SARS-CoV-2 Detection Kit is an ideal solution for organizations and institutions looking for a rapid and reliable solution for the detection of SARS-CoV-2 virus. It can be used in healthcare settings, laboratories, and other research institutes to identify the presence of SARS-CoV-2 virus in human samples, and it can also be used in the diagnosis and treatment of COVID-19 patients. The kit is suitable for use in public health surveillance programs to monitor the spread of the virus, and it can also be used for the rapid diagnosis and monitoring of SARS-CoV-2 infections in high-risk areas.
The Amp-future bio SARS-CoV-2 Detection Kit is a reliable and cost-effective solution for the detection and identification of SARS-CoV-2 virus. It is manufactured in China, and it has been developed following the highest standards of quality assurance. This kit is an indispensable tool for organizations and institutions looking for a rapid and reliable solution for the detection of SARS-CoV-2 virus in human samples.
Support and Services:
At , our goal is to provide customers with the best SARS-CoV-2 Detection Kit available. To ensure customer satisfaction, we provide a variety of technical support and service options.
Customer Support: Our customer support representatives are available to answer any questions you may have about the SARS-CoV-2 Detection Kit, including its features, compatibility, and installation.
Online Documentation: Our online documentation provides detailed instructions on how to use the SARS-CoV-2 Detection Kit, as well as troubleshooting tips and other helpful information.
Warranty and Returns: We provide a limited warranty on our SARS-CoV-2 Detection Kit to protect our customers against manufacturer defects or malfunctions. If you experience any issues with your product, please contact us for assistance.
Software Updates: We regularly release software updates for the SARS-CoV-2 Detection Kit to ensure optimal performance. You can easily download and install the latest updates from our website.
If you have any questions or need further assistance, please contact us at and one of our representatives will be happy to help.
FAQ:
Q1: What is SARS-CoV-2 Detection Kit?
A1: SARS-CoV-2 Detection Kit is a product produced by Amp-future bio, which is originated from China. It is used for detecting the presence of SARS-CoV-2 virus in a sample.
Q2: What is the principle of SARS-CoV-2 Detection Kit?
A2: SARS-CoV-2 Detection Kit utilizes the PCR (polymerase chain reaction) technology to amplify small amounts of the virus’s genetic material, allowing it to be detected with high accuracy.
Q3: What type of sample can be tested with SARS-CoV-2 Detection Kit?
A3: SARS-CoV-2 Detection Kit is suitable for testing samples such as nasal swab, nasopharyngeal swab, sputum, saliva, throat swab and bronchoalveolar lavage fluid.
Document
Introducing the SARS-CoV-2 Detection Kit – the perfect solution for quick and accurate COVID-19 infection testing. This revolutionary kit has been designed to detect the presence of SARS-CoV-2 in Nasopharyngeal swabs, providing you with a reliable and convenient way to determine whether individuals have been infected with the virus. With the ability to perform 48 tests per kit, our detection kit is the perfect solution for rapid and accurate testing.
Our detection kit is designed for maximum accuracy and reliability, with components that must be stored at -20°C to ensure optimal performance. Our convenient shipping process ensures that the kit arrives frozen and ready for use, so you can rest assured that you’ll have the results you need as quickly as possible.
The SARS-CoV-2 Detection Kit is the most reliable and accurate COVID-19 infection testing solution on the market. With its convenient packaging and easy-to-use design, our detection kit is the perfect solution for any medical facility looking to quickly and accurately test for the presence of SARS-CoV-2.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main Functions
IVD5412 precast kit for kingfisher Flex
Applications
RT-PCR，PCR，NGS
Products
Viral DNA / RNA, body cell DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technolog
Process method
Manual or automatic
Sample type
Sample amount
200μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
Cat.No
Reagent
IVD5412-F-96
PK/Carrier RNA
1x 24 mg/Bottle
Protease Dissolve Buffer Blue
1.8 ml/Bottle
Tip
1
Sample Plate (DW Plate)
500µl Buffer MLB/Well
1
Wash 1 Plate (DW Plate)
500µl Buffer MW1/Well
1
Wash 2 Plate (DW Plate)
500µl Buffer CW/Well
1
Beads Plate (DW Plate)
500µl CW & 20µl MPN/Well
1
Elute Plate (KF Plate)
90µl NFW/Well
1
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Document
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic Cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
Key Features:
Heat-labile – Completely and irreversibly inactivated at 55°C
Contamination control – ideal in applications below
Use of Cod UNG makes contamination control possible in RT-PCR
Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
High purity enzyme, tested free of contaminating nucleases
There are several commercially available Uracil-DNA glycosylases on the market today. Most of them are of bacterial origin and work well if you have no intention to further analyze the PCR products post-PCR. However, if you want to store your PCR products for downstream analysis such as cloning and sequencing, the reactivation of UNG and subsequent degradation of your PCR products are a problem with most of the commercially available UNGs. Cod UNG from ArcticZymes is completely and irreversibly inactivated by heat thus ensuring that sample integrity is maintained long-term regardless of storage conditions.
This is illustrated in figure 1, below
Figures
Properties
Recommended Protocols
1. Contamination control in PCR, qPCR and one-step RT-qPCR
Cod UNG works in all commercially available master mixes.
Be sure that you have used dUTP containing dNTP mixes in your previous PCR experiments.
Add 0.2 U Cod UNG directly to your 20 µl PCR reaction.
pre-incubate for 5 min at room temperature.
For RT-qPCR, reverse transcribe your RNA at 50-55°C.
Run your PCR.
Store your PCR product at -20°C or 4°C degrees.
2. Contamination control in RT-LAMP
Cod UNG is ideal for contamination control in RT-LAMP. One unit of Cod UNG per 30 μl reaction is sufficient for removing even high concentrations of carry-over contamination.
Ensure that you use dNTP mixes containing dUTP in your experiments.
Check that the RT-LAMP reaction is compatible with dUTP by running side-by-side reactions containing different ratios of dUTP to dUTP (100% dUTP, 90% dUTP, 80% dUTP and 0% dUTP).
Add 1 U Cod UNG directly to your 30 µl RT-LAMP reaction.
Prepare the reaction mix on ice.
Analyze your RNA at 65°C, no preincubation is necessary.
Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic Cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
