This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Detail
Introduction
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Clinical tissues, cells, lymphocytes
Sample amount
Tissue: <20 mgCells: <5 x 106
Yield
2-50μg
Elution volume
≥30μl
Time per run
≤25 minutes
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
Advantages
Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – the whole extraction only takes 15-25 minutes
Nontoxic – no toxic phenol chloroform extraction is required in the extraction
Kit Contents
Contents
IVD4121
Purification Times
50 Preps
HiPure DNA Mini Column Ⅱ
50
HiPure RNA Mini Columns
50
2ml Collection Tubes
150
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Buffer
6 ml
Buffer RTL
40 ml
RNA Digestion Buffer
15 ml
Buffer RWC*
20 ml
Buffer RW2*
20 ml
Nuclease Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Other Products
DNA Extract Nucleic Acid Reagent For PCR 12 Months Shelf Life
Product Info
Document
Product Info
Product Description
High-Quality Nucleic Acid Reagent for PCR
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
Features:
Product Name: Nucleic Acid Extraction Reagent
Form: Liquid
Brand: Amp-future Bio
Composition: DNA
Shelf Life: 12 Months
DNA RNA Extraction Kit
Automated Nucleic Acid Extractor
Technical Parameters:
Product Name
Technical Parameters
Nucleic Acid Extraction Reagent
Application: DNA Extract Form: Liquid Shelf Life: 12 Months Brand: Amp-future Bio Automated Nucleic Acid Extractor, DNA Extraction Kit, Automated Nucleic Acid Extractor
Applications:
Amp-future Bio Nucleic Acid Extraction Reagent is a liquid reagent specially formulated for automated nucleic acid extraction. It is widely used in DNA/RNA extraction from various sample matrixes, such as blood, tissue, cells, bacteria, etc. It ensures high-quality nucleic acid extraction in a short period of time. The reagent is produced in China and has a shelf life of 12 months.
Amp-future Bio Nucleic Acid Extraction Reagent is suitable for use with the Automated Nucleic Acid Extractor. It is highly efficient and reliable, allowing for fast and accurate nucleic acid extraction. The reagent is also suitable for use with the DNA Extraction Kit, which makes it ideal for clinical laboratories. It provides superior nucleic acid extraction performance.
Amp-future Bio Nucleic Acid Extraction Reagent is an essential product for laboratories that need to extract nucleic acids from various sample matrixes. It is highly reliable and efficient, providing superior results in a short period of time. The reagent is also suitable for use with the Automated Nucleic Acid Extractor and the DNA Extraction Kit. It is a reliable and cost-effective product for laboratories that require high quality nucleic acid extraction.
Customization:
Customized Nucleic Acid Reagent Service
Amp-future bio provides custom nucleic acid reagent services to meet your specific needs. We specialize in the production of DNA/RNA extraction reagents for automated nucleic acid extraction, purification and other related applications.
Brand Name: Amp-future bio
Place of Origin: China
Product Name: Nucleic Acid Extraction Reagent
Brand: Amp-future Bio
Form: Liquid
Shelf Life: 12 Months
Application: DNA Extract
Our products are suitable for DNA extraction, automated nucleic acid extraction, nucleic acid purification, and other related applications. We are confident that our products can meet the highest quality standards and provide the best performance. We are dedicated to providing the best customer service and technical support for our customers.
Support and Services:
Our team of experts provide the highest quality technical support and services for Nucleic Acid Reagents products.
Technical Support
Our technical support staff are available to assist you with any questions or troubleshooting related to the use of Nucleic Acid Reagents. Our team can provide guidance on the proper use and handling of our products, as well as any other technical questions. We offer technical support via phone, email, and online chat.
Services
We also offer a variety of services to support our customers in the use of Nucleic Acid Reagents. These services include:
Troubleshooting and technical advice
Installation and maintenance of products
Product training and certification
Custom protocols and procedures
Document
Amp-future Bio’s Nucleic Acid Extraction Reagent is a high quality and reliable DNA extraction kit that is specifically designed for automated nucleic acid extraction. It has a shelf life of 12 months and comes in a convenient liquid form. This product is the perfect choice for researchers and laboratories that require fast and accurate results, as it offers a highly efficient solution for extracting nucleic acids from a variety of biological samples. It is easy to use and can be used in either manual or automated protocols. The reagent contains a broad spectrum of components that are essential for the successful isolation and extraction of nucleic acids. With its automated nucleic acid extractor, it ensures that all samples are processed with maximum efficiency and accuracy.
The process of automatically removing seals from plates has become more popular due to the repetitive task of removing plate seals, which can potentially lead to cross contamination, repetitive motion injuries & plate/seal damage.
Application: Automatic & Manual MicroPlate Seal Removal
Suitable for use in the Automated Plate Seal Remover (formally XPeel)
Document
Overview The process of automatically removing seals from plates has become more popular due to the repetitive task of removing plate seals, which can potentially lead to cross contamination, repetitive motion injuries & plate/seal damage. Application: Automatic & Manual MicroPlate Seal Removal Suitable for use in the Automated Plate Seal Remover (formally XPeel)
Lyophilized RNA Amplification Kit Below 20 Degree Format Easy And Efficient
Product Info
Document
Product Info
Product Description
Lyophilized RNA Amplification Kit -20°C Format Easy And Efficient
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
RNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
