This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Detail
Introduction
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Rapidly Extract viral DNA/RNA from 200μl non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, etc.
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Fast – column purification without PK digestion
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
Contents
IVD4175
Purification Times
100 Preps
HiPure Viral Column
100
2ml Collection Tubes
100
PK/Carrier RNA
50 mg/310μg
Protease Dissolve Buffer
5 ml
Buffer VLE
42 ml
Buffer CE
60 ml
RNase Free Water
15 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Other Products
Plasma/Serum RNA Purification Kits
Product Info
Document
Product Info
Overview
Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma/serum input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into a flexible elution volume
High quality, purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Compatible with Streck Cell-Free RNA BCT® Tubes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Background
Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.
Plasma/Serum RNA Purification Mini Kit
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL.
Plasma/Serum RNA Purification Midi Kit
This utilizes a two column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.
Plasma/Serum RNA Purification Maxi Kit
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.
All sizes, including miRNA and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields*
Variable depending on specimen
*Please check page 7 of the Product Insert for Average Plasma/Serum Yields and Common RNA Quantification Methods.
This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.
All reagents stable for > 12 months after preparation
Simple format
Very specific
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.
1. Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.
2. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.
3. Automatic lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
4. Adapters are available by experiment requirements.
5. 3 tiers protection steel cover, safe and reliable.
6. TUV CE EMC certificate.
TD5A Technical Parameter:
Max. Speed
5000rpm
Max. RCF
4730×g
Max. Capacity
4×300ml
Time Range
0~99min
RPM/RCF Convert
Yes
Noise (dB)
≤ 58
Temperature
Normal
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
/
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
620×450×355mm
Net Weight(Kg)
52KG
Certificates
CE,ISO & Calibration report are available
Matched Rotor for TD5A
Order NO.
Rotor Type
Max speed(r/min)
Volume (ml)
Max RCF(*g)
5A-1
Swing rotor (round cup)
4000
4×300ml
3180
5A-2
Swing rotor (square cup)
4000
4×300ml
3150
5A-3
Swing rotor
4000
4×24×7ml
3150
4000
4×24×5ml
2750
4000
4×18×10ml
3140
5A-4
Microplate rotor
4000
2×4×96 well
2910
5A-5
Microplate rotor
4000
2×4×48 well
2300
5A-6
Swing rotor
5000
4×1×50ml
4730
5000
4×1×100ml
4730
5A-7
Swing rotor
4000
4×2×50ml
3200
4000
4×2×100ml
3020
4000
4×4×15ml
3200
4000
4×6×15ml
3200
4000
4×8×15ml
3200
4000
4×12×5ml vacuum tube
2480
4000
4×12×7ml vacuum tube
2760
4000
4×12×10ml vacuum tube
2790
4000
4×8×10/7/5ml vacuum tube
2790
4000
4×6×10/7/5ml vacuum tube
2790
4000
4×4×10/7/5ml vacuum tube
2790
5A-8
Fixed Rotor
5000
6×15ml
2540
5A-9
Fixed Rotor
5000
12×15ml
3080
5A-10
Fixed Rotor
5000
24×15ml
3500
5A-11
Fixed Rotor
5000
30×15ml
3830
5A-12
Fixed Rotor
5000
4×50ml
2520
5A-13
Fixed Rotor
5000
6×50ml
2850
5A-14
Fixed Rotor
5000
12×50ml
3860
5A-15
Fixed Rotor
4000
24×50ml
2970
5A-16
Fixed Rotor
5000
4×100ml
2630
5A-17
Fixed Rotor
5000
6×100ml
3130
5A-18
Fixed Rotor
4000
12×100ml
2970
Document
TD5A centrifuge Max Capacity 4x300ml swing out rotor, max speed 5000rpm, can hold 96 vacuum tubes