Introduction
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Details
Specifications
| Features | Specifications |
| Main Functions | Rapidly Extract viral DNA/RNA from 200μl non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, etc. |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA, body cell DNA / RNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Plasma, serum, effusion, urine, fecal suspension supernatant |
| Sample amount | 200μl |
| Elution volume | ≥30μl |
| Time per run | |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
- Fast – column purification without PK digestion
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
| Contents | IVD4175 |
| Purification Times | 100 Preps |
| HiPure Viral Column | 100 |
| 2ml Collection Tubes | 100 |
| PK/Carrier RNA | 50 mg/310μg |
| Protease Dissolve Buffer | 5 ml |
| Buffer VLE | 42 ml |
| Buffer CE | 60 ml |
| RNase Free Water | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
