Introduction
This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-6ml plasma, serum, body fluids |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 1-6ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – without labour
- High purity – OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0
Kit Contents
| Contents | IVD5435 |
| Purification Times | 50 |
| MagPure Particles F | 14 ml |
| Carrier RNA | 310 μg |
| Proteinase K | 240 mg |
| Protease Dissolve Buffer | 15 ml |
| Buffer SDS | 15 ml |
| Buffer MLK | 500 ml |
| Buffer MAW1 | 250 ml |
| Buffer MW2* | 50 ml |
| Elution Buffer | 60 ml |
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
