Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
MycoSEQ Mycoplasma Detection Kit（Real-Time PCR）
Product Info
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Product Info
Description
Mycoplasma is the smallest and simplest prokaryotic organism. There is a risk of mycoplasma contamination during cell culture, and mycoplasma contamination of cells has become a common problem worldwide. Mycoplasma contamination may seriously affect the state of cells, change the gene expression and metabolic characteristics of cells, lead to slow cell growth, abnormal differentiation and death, and seriously affect cell function. Bacteria, yeast or mold contamination in cell culture can be seen under an optical microscope, but mycoplasma contamination is usually not visible under an optical microscope and must be detected by specific detection methods. Common methods for detecting mycoplasma contamination include mycoplasma isolation and culture, special biochemical tests such as ELISA and luminescence, and DNA fluorescent staining detection. Among the above detection methods, most of the operation steps are relatively cumbersome, the sensitivity is not high, the mycoplasma types cannot be distinguished, special instruments are required, or the time required is long. The qPCR method is relatively simple and convenient to operate, and the results can be obtained in 2 hours.
Features of MycoSEQ Mycoplasma Detection Kit
• Provide results in less than 2 hours. Good negative and positive controls. • No live mycoplasma required: The MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require the use of live mycoplasma for validation • Can be used with gDNA, inactivated mycoplasma, or live mycoplasma • Can be used as an alternative to the standard 28-day culture test • Can be used in conjunction with culture-based methods to provide preliminary results while awaiting the 28-day test results
The MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious drug, impurity, and contaminant detection during biopharmaceutical manufacturing. The entire workflow includes the sample preparation kit, which provides a manual sample preparation method, which together with the qPCR analysis can provide results in 2 hours. (Please note that complex matrices may require additional upfront processing steps as described in the various available protocols.)
Contents and Storage
• Mycoplasma qPCR MIX, store at -15°C to -25°C, store at 2°C to 8°C after first use, protected from light. • Mycoplasma Positive Control, -15°C to -25°C. • Mycoplasma Negative Control, store at -15°C to -25°C, store at 2°C to 8°C after first use.
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Note: Price not include shipment & duty, contact us to get full quote.
DuckyBio’s Mycoplasma PCR Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for detecting mycoplasma contamination in biological materials such as cultured cells. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in European Pharmacopoeia (E.P. 2.6.7, 2007), US Pharmacopoeia (US63) and Japanese Pharmacopoeia. When used with a suitable sample preparation method, the MycoSEQ Plus kit can detect less than 5 CFU/mL.
6-Formyl-2-pyridine-PEG4-DBCO is a bifunctional PEG linker featuring an aldehyde and a DBCO function. DBCO is a strained cyclooctyne which reacts with azide groups on target molecules while the aldehyde group is a versatile electrophile that is reactive towards amines, hydroxylamines, and hydrazines.
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6-Formyl-2-pyridine-PEG4-DBCO is a bifunctional PEG linker featuring an aldehyde and a DBCO function. DBCO is a strained cyclooctyne which reacts with azide groups on target molecules while the aldehyde group is a versatile electrophile that is reactive towards amines, hydroxylamines, and hydrazines.
Permagen’s 16 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 16 tubes
Contains the strongest magnets out of all of our Microfuge racks
Tubes are pushed 14mm between centers allowing the use of an 8-channel, adjustable head pipette. 8 tubes per side also make for easy transition to and from microplates
Accommodates many common 1.5 mL Microcentrifuge and some 2.0 mL tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
