L-Arginine/Urea/Ammonia Assay Kit

K-LARGE

SKU: 700004309

50 Assays per kit

Content:	50 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	Ammonia, L-Arginine, Nitrogen, Urea, YAN
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Decrease
Linear Range:	1.0 to 35 mg of L-arginine, 0.2 to 7.0 μg of ammonia and 0.3 to 14 µg of urea per assay
Limit of Detection:	0.07 mg/L (ammonia), 0.13 mg/L (urea), 0.37 mg/L (L-arginine)
Reaction Time (min):	~ 20 min [ammonia (2 min), urea (6 min), L-arginine (7 min)]
Application examples:	Grape juice, wine must, wine and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Improved method

The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuant^TM Wave Spectrophotometer (D-MQWAVE).

Display all of our nitrogen assay kit products.

Advantages

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
Improved assay format
Very rapid reactions due to use of uninhibited glutamate dehydrogenase
All enzymes supplied as stabilised suspensions
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

Overview

Purify amplified DNA ranging from 100 bp -15,000 bp in size

Fast and efficient spin column format

Available in a 50 prep size and a 250 prep size

Also available in 96 well format

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

Figure 1 / 2

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Kit Specifications – 96-well
Binding Capacity Per Well	15 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	100 – 15,000 bp
Average DNA Recovery	> 90%
Average Primer Reoval	> 90%
Minimum Elution Volume	50 μL
Time to Complete 96 Purifications	20 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 14400 (50 preps)	Cat. 45700 (250 preps)	Cat. 24800 (192 preps)
Binding Buffer C	30 mL	5 x 30 mL	3 x 30 mL
Wash Solution A	12 mL	2 x 20 mL	2 x 38 mL
Elution Buffer B	8 mL	2 x 30 mL	2 x 15 mL
Spin Columns	50	250	–
Collection Tubes	50	250	–
96-Well Plate	–	–	2
Adhesive Tape	–	–	4
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	250	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	2

L-Arginine/Urea/Ammonia Assay Kit

Detail

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