Laboratory Large Capacity Freezer Dryer/ Lyophilizer SF7009,SF9009,SF7012,SF9012

Model:
SF7009,SF9009,SF7012,SF9012

Brand:
Yingtai

Detail

Techinical parameter

Item No	SF7009	SF9009	SF7012	SF9012
Ice condenser capacity	9kg		12kg
Ice condenser temperature	-70 C°	90℃	-70 C°	-90℃
Cold trap volume	15L		20L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	●φ260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optional
Vacuum pump	Extreme vacuum 510mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filter	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment	Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	1-48 ports/optiona
Eutectic point judgment	Support/Optional
Data export analysis	Support/Optional
Power	0.9kw	1.5kw	1kw	1.6KW
Size	5255701080mm

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Lyophilized RNA Amplification Kit Below 20 Degree Format Easy And Efficient

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Product Description

Lyophilized RNA Amplification Kit -20°C Format Easy And Efficient

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Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

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Stool Nucleic Acid Collection and Preservation Devices Dx

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Overview

CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro workflows
No need to immediately process samples
Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
Ship stool samples at room temperature safely
Compatible with most DNA/RNA isolation methods

Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx are designed for the collection and preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Devices Dx – 50 contains 50 individual Stool Nucleic Acid Collection and Preservation Devices Dx. Each Stool Nucleic Acid Collection and Preservation Device Dx consists of Norgen’s Stool Nucleic Acid Collection and Preservation Tube Dx containing Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Nucleic Acid Collection and Preservation Tube Dx is subsequently sent to the laboratory for DNA and/or RNA isolation and analysis. The stool DNA in preserved samples is stable for more than 2 years at room temperature, and the stool RNA in preserved samples is stable for 7 days at room temperature. DNA can be isolated from the preserved stool samples using Norgen’s Stool DNA Isolation Kit Dx (Cat. Dx27600) and RNA can be isolated from the preserved stool samples using Norgen’s Stool Total RNA Purification Kit Dx (Cat. Dx49500). These tubes are ideal for collecting and preserving DNA and RNA samples for in vitro diagnostic use for medical purposes.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

Figure 1 / 9

Figure 1. Norgen's Best-In-Class Preservation

Figure 1. Norgen's Best-In-Class Preservation

Figure 2. High Integrity of Preserved DNA and RNA

Figure 3. Stability of DNA

Figure 4. DNA is Stable over a Wide Range of Temperatures

Figure 5. Stability of Genes over a 7 Day Period

Figure 6. Hierarchical Clustering of Stool Samples at Phylum Level

Figure 7. Difference In Top 5 Abundant Microorganisms at Phylum Level

Figure 8. Relative Abundance of Bacterial Genera

Figure 9. Correlation between duplicate bacterial metagenomic reads

Click for expanded view

Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will be vary depending on the samples

Stool Nucleic Acid Collection and Preservation Devices Dx – 50 Contents:

Kit Components	Cat. Dx45660
Stool Nucleic Acid Collection and Preservation Devices Dx	50
Product Insert	1

Stool Nucleic Acid Collection and Preservation Device Dx Contents:

Kit Components	Contents
Stool Nucleic Acid Collection and Preservation Tube Dx filled with preservative	1
Insert Card	1

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Urine DNA Isolation Kits

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Overview

Rapid isolation of both small and large species of DNA from urine
Convenient spin column format
Effective removal of PCR inhibitors
Purified DNA is highly suited to sensitive downstream applications
Allows for the purification of viral DNA from urine

Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.

This kit is fully compatible with Norgen’s Urine Collection and Preservation Tubes.

Urine DNA Isolation Kit

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.

Urine DNA Isolation Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.

Urine DNA Isolation Maxi Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.

Background

DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.

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Supporting Data

Figure 1 / 8

Figure 1. A Typical Agarose Gel Showing Total Urinary DNA

Figure 1. A Typical Agarose Gel Showing Total Urinary DNA

Figure 2. Isolation and Detection of DNA from 1.75 mL Urine Samples

Figure 3. Circulating DNA Isolated from Urine

Figure 4. Isolation of Total Urinary DNA from Different Urine Volumes

Figure 5. Isolation and Detection of DNA from Different Urine Volumes

Figure 6. Highly Sensitive Isolation of Viral DNA

Figure 7. Total Urinary DNA Isolated from Different Urine Volumes

Figure 8. Linear Decrease of Ct Values with Increasing Urine Input

Click for expanded view

Kit Specifications – Spin Column
Minimum Urine Input	3 mL
Maximum Urine Input	25 mL
Time to Complete 10 Purifications	< 30 minutes
Size of Urine DNA Purified	Large (> 1 kb) and small (150-250 bp)

Kit Specifications – 96-well
Urine Input Volume	3 – 25 mL
Elution Volume	1st Elution 100 μL 2nd Elution 100 μL
Size of DNA Purified	Large (> 1 kb) and small (150-250 bp)
Time to Complete 96 Purifications	< 1 hour

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Slurry B1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry B1 contains a grey resin that will not dissolve when warmed.

Component	Cat. 18100 (50 preps)	Cat. 48800 (50 preps)	Cat. 50100 (50 preps)
Binding Solution K	15 mL	–	–
Slurry B1	–	18 mL	18 mL
Proteinase K in Storage Buffer	2 mL	–	–
Pronase K in Storage Buffer	2 mL	–	–
Soluton WN	9 mL	–	–
Binding Buffer A	–	–	50 mL
Lysis Buffer A	–	30 mL	30 mL
Wash Solution A	–	38 mL	38 mL
Wash Solution B	30 mL	–	–
Wash Solution D	9 mL	–	–
Binding Solution K	15 mL	–	–
Elution Buffer B	15 mL	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Filter Spin Columns	–	50	50
Collection Tubes	50	50	50
Elution Tubes (1.7 mL)	100	100	100
Product Insert	1	1	1

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