Laboratory Large Capacity Freezer Dryer/ Lyophilizer SF7009,SF9009,SF7012,SF9012

Model:
SF7009,SF9009,SF7012,SF9012

Brand:
Yingtai

Detail

Techinical parameter

Item No	SF7009	SF9009	SF7012	SF9012
Ice condenser capacity	9kg		12kg
Ice condenser temperature	-70 C°	90℃	-70 C°	-90℃
Cold trap volume	15L		20L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	●φ260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optional
Vacuum pump	Extreme vacuum 510mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filter	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment	Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	1-48 ports/optiona
Eutectic point judgment	Support/Optional
Data export analysis	Support/Optional
Power	0.9kw	1.5kw	1kw	1.6KW
Size	5255701080mm

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MINI-3 Microplate Centrifuge

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RNA/DNA Purification KitsOverview

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RNA/DNA Purification Micro Kit

Overview

Sequentially isolate and purify total RNA and DNA from a single sample
Two column system: one for DNA and one for RNA
The RNA column is for the purification of total RNA including microRNA
No need to split the lysate, or to use phenol or precipitation methods
Rapid and efficient spin column procedure – it takes only 30 minutes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a rapid method for the isolation and purification of total RNA and DNA sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. The lysate is passed over two columns: 1) a DNA column and 2) an RNA column. Total RNA of all sizes is purified, including microRNA. Both DNA and RNA are of the highest purity and yield.

These kits are ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kits are especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as they eliminate the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications

RNA/DNA Purification Kit (Spin Column)

Maximum column binding capacity of 50 μg for RNA and 20 μg for DNA.

Workflow

RNA/DNA Purification Micro Kit (Micro)

The purified RNA and DNA fractions can be eluted in as little as 20 μL. Ideal for cell number inputs of 500,000 and as little as 5 cells. Maximum column binding capacity of 35 μg for RNA and 10 μg for DNA.

Details

Supporting Data

Figure 1 / 4

Figure 1. Recovery of True Total RNA including microRNA from Hamster Liver using Norgen's RNA/DNA Purification Kit

Figure 1. Recovery of True Total RNA including microRNA from Hamster Liver using Norgen's RNA/DNA Purification Kit

Figure 2. Recovery of Intact, High Quality Genomic DNA from Hamster Liver using Norgen's RNA/DNA Purification Kit

Figure 3. Recovery of True Total RNA including microRNA from Hamster Liver using Norgen's RNA/DNA Purification Micro Kit

Figure 4. Recovery of Intact, High Quality Genomic DNA from Hamster Liver using Norgen's RNA/DNA Purification Micro Kit

Click for expanded view

Kit Specifications
Maximum Column Binding Capacity	50 μg for RNA 20 μg for DNA
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues	5 x 10⁶ cells 25 mg (for most tissues)** 200 μL 1 x 10⁹ cells 1 x 10⁸ cells 50 mg 50 mg
Time to Complete 10 Purifications	30 minutes
Average Yield*: HEK 293 Cells (1 x 10⁶ cells) HEK 293 Cells (1 x 10⁶ cells) Liver (15 mg) Liver (15 mg)	10-15 μg RNA 2-4 μg DNA 30-35 μg RNA 4-6 μg DNA

*Average Yield will vary depending upon a number of factors including species, growth conditions used, and development stage.

**Tissue inputs of up to 40 mg may be used, however for inputs greater than the recommended 25 mg, cross-contamination of the RNA and DNA fractions is possible.

Storage Conditions and Product Stability
Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48700 (50 preps)	Cat. 50300 (50 preps)
Buffer SKP	40 mL	40 mL
Wash Solution A	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL
Elution Solution A	6 mL	6 mL
Elution Buffer F	15 mL	6 mL
RNase-Free Water	40 mL	40 mL
Proteinase K	2 x 12 mg	2 x 12 mg
gDNA Purification Columns	50	–
gDNA Purification Micro Columns	–	50
RNA Purification Columns	50	–
RNA Purification Micro Columns	–	50
Collection Tubes	100	100
Elution Tubes (1.7 mL)	100	100
Product Insert	1	1

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Plasmid DNA Kits

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Product Info

Overview

Isolate high quality, high yield plasmid DNA
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Details

Supporting Data

Figure 1 / 5

Figure 1. High Yield DNA from Small Cultures

Figure 1. High Yield DNA from Small Cultures

Figure 2. High Accuracy Sequencing

Figure 3. High Quality of Plasmid DNA

Figure 4. High DNA Yield

Figure 5. Full Compatibility with Digests

Click for expanded view

Kit Specifications
Column Binding Capacity	1.5 mg
Average Yield from 100 mL Culture*	0.4 – 1.0 mg
Final Endotoxin Levels	< 0.1 EU/µg DNA
Time to Complete 4 Purifications	1.5 hours
Size of Plasmids Purified	Up to 13,000 bp

* Depends on high-copy DNA and low copy DNA

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

Component	Cat. 13300 (50 preps)	Cat. 46400 (250 preps)	Cat. 46500 (4 preps)	Cat. 46600 (20 preps)	Cat. 60300 (50 preps)	Cat. 63000 (192 preps)
Resuspension Solution AZ	12 mL	60 mL	20 mL	100 mL	12 mL	2 x 20 mL
Lysis Buffer N	40 mL	80 mL	40 mL	2 x 80 mL	40 mL	2 x 40 mL
Buffer TN	20 mL	130 mL	55 mL	2 x 130 mL	20 mL	1 x 55 mL 1 x 20 mL
Wash Solution E	12 mL	2 x 18 mL	–	–	–	–
Elution Buffer K	8 mL	30 mL	–	–	8 mL	2 x 8 mL
Wash Solution J	–	–	25 mL	3 x 25 mL	–	–
Elution Buffer J	–	–	24 mL	120 mL	–	–
RNase A	1 vial	1 vial	1 vial	1 vial	1 vial	1 vial
Magnetic Bead Suspension	–	–	–	–	1 x 1.1 mL	4 x 1.1 mL
Spin Columns	50	250	–	–	–	–
Collection Tubes	50	250	–	–	–	–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)	–	–	4	20	–	–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)	–	–	4	20	–	–
96-Well Plate	–	–	–	–	–	2
Elution Tubes (1.7 mL)	50	250	–	–	50	–
Elution Tubes (50 mL)	–	–	4	20
96-Well Elution Plate	–	–	–	–	–	2
Adhesive Tape	–	–	–	–	–	2
Product Insert	1	1	1	1	1	1

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