Laboratory Square Cavity Front Door Freeze Dryer/ Lyophilizer

Overview

• Detection kits for H5N1
• Available in TaqMan format for analysis

Influenza virus infection of birds, humans and other animals is a major public health problem worldwide. Influenza viruses are classified as either type A, B or C based on differences in their nucleoproteins and matrix proteins. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease and epidemics. The different types can be further classified into subtypes based on antigenic differences in two surface glycoproteins; hemagglutinin and neuroamidase. All known subtypes of influenza A can be found in birds (H1-H16, N1-N9), while a limited number of the subtypes have been found in humans (H1-H3, N1 and N2). However, over the past few years, various subtypes of Influenza A viruses, including H5N1, have been reported to infect humans (WHO, 2006). In addition, the coexistence of human influenza viruses and avian influenza viruses may provide an opportunity for genetic material to be exchanged between these viruses. This could potentially create a new virulent influenza strain that is easily transmissible and lethal to humans (Food Safety Research Information Office, 2006). Thus, there is the need for sensitive diagnostic tests to allow for the rapid and early detection of these H5 influenza virus infections, to help reduce the risk of epidemics or pandemics in both animals and humans.

H5N1 TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

H5N1 TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM35450 (100 preps)	Cat. TM35410 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
H5N1 Primer & Probe Mix	280 μL	280 μL
H5N1 Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

N-(Propargyl-PEG2)-PEG3-N-Boc is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The Boc group can be deprotected under acidic conditions. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose.

N-(Propargyl-PEG2)-PEG3-N-Boc is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The Boc group can be deprotected under acidic conditions. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose.

N-Boc-N-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are combined together at a Boc-protected secondary amine. Terminal alkynes are most commonly used in copper click chemistry with azides to form stable triazoles with the target molecule. The secondary amine may be deprotected under acidic conditions to allow for alkylation at that position, increasing the molecule’s structural complexity. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

N-Boc-N-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are combined together at a Boc-protected secondary amine. Terminal alkynes are most commonly used in copper click chemistry with azides to form stable triazoles with the target molecule. The secondary amine may be deprotected under acidic conditions to allow for alkylation at that position, increasing the molecule’s structural complexity. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.