Name of Product
Lactobacillus brevis
Catalog Number
MGLBr Z
Short Info
GenLine discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 -10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Detail
Name of Product
Lactobacillus brevis
Catalog Number
MGLBr Z
Short Info
GenLine discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 -10^5 cfu/mL
Brief Instructions
The PCR reagents and the samples are prepared.
After the addition of the sample to the PCR reagents, the PCR is started.
The resulting PCR products are detected by a simple lateral flow test Strip.
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Other Products
Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
Product Info
Document
Product Info
Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
The kits were developed for plasmid miniprep directly from bacterial cultures. 100% centrifuge-free; NO vacuum needed; No column needed.
Plasmid MiniPrep Kit (Magnetic Beads), Cat.# 50012 – Use 2 ml of bacteria culture directly
Plasmid MiniPrep High Throughput Kit (Magnetic Beads), Cat.# 50011 – High throughput: Use 0.2 ml of bacteria culture directly with 96-well plates – Low throughput: Use 0.2 ml of bacteria culture directly with 1.5 ml tubes
The kits can be used for both high copy and low copy numbers of plasmid DNA. With BioDynami’s proprietary magnetic beads technology, the kits eliminate the requirement of centrifuge, vacuum, and column. Our magnetic beads provide a robust and reliable tool for both high throughput and low throughput applications of plasmid DNA isolation with high binding capacity and fast magnetic response time.
The High Through kit (Cat.# 50011) can be used for both high throughput and low throughput extraction of plasmid DNA. Up to 96 samples can be extracted simultaneously when using a 96-well plate.
Yield of plasmid DNA may vary dependent on the bacteria strain, plasmid type, copy numbers, and growth conditions etc. DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, molecular cloning, restriction enzymatic digestion, transfection, and transformation etc. The isolated plasmid DNA with the magnetic beads is free of contaminations such as RNA, bacterial DNA, proteins, and other impurities.
Plasmid DNA were loaded on 1% of agarose gel after extraction.
Comparison of workflows of Magnetic Beads based kits
Features
100% centrifuge-free
Culture medium can be used directly without centrifuge to pellet the bacteria
Simple
No centrifuge
No column
No vacuum
Flexible applications with simplified miniprep protocol
High throughput: 96-well plates with 0.2 ml of each culture (Cat.# 50011)
Low throughput: 1.5 ml tubes with 0.2 ml of each culture (Cat.# 50011)
Low throughput: 1.5 ml tubes with 2 ml of each culture (Cat.# 50012)
Rapid and simple procedure to generate digested peptides
Simultaneous digestion, purification and concentration at once
Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry
Peptides are ready for applications such as mass spectrometry and SDS-PAGE
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format. Trypsin is added to the protein sample and bound to the column. Salts are washed away and the trypsin is then activated to digest proteins. Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation). The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.
