Quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range
Compatible with any Real-Time PCR system
RNA is accurately quantified using a standard curve constructed from the provided RNA standard
Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard.
Storage Conditions Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
Component
Cat. 58900 (48 reactions)
microScript microRNA Enzyme Mix
20 μL
2x microScript Reverse Transcriptase Reaction Mix
0.5 mL
2X Real-Time PCR Master Mix
1 mL
RNA Quantification Primer Set Mix
200 μL
Quantified RNA Standard 50 pg/μL
2 x 60 μL
Nuclease-Free Water
3 x 1.25 mL
Product Insert
1
Other Products
【RC1000】EzRNA™ RNA Capping System, 50 RXN
Product Info
Document
Product Info
Description
The EzRNA™ RNA Capping System is a user-friendly product for post-transcriptional RNA modification. Both Vaccinia Capping Enzyme and 2’-O-Methyltransferase are included in the package, which are able to perform in a single reaction. The Vaccinia Capping Enzyme attach 7-methylguanylate cap (m7Gppp, Cap-0) to the 5′ end of RNA to form m7Gppp5’N-RNA (Cap-0 RNA). The 2′-O-methyltransferase utilizes Cap-0 RNA as a substrate, employing S-adenosine methionine (SAM) as a methyl donor to methylate 2′ -OH of the first nucleotide at the 5′ end of Cap-0 RNA, resulting in the formation of Cap-1 RNA.
Features
2’-O-Methyltransferase included for Cap-1 RNA
High capping efficiency
High stability
RNase inhibitor is included to enhance the stability of capping reaction
Application
Generation of 5’Cap-0 (m7Gppp) and Cap-1 (m7GpppNm-) RNA by enzymatic reaction
mRNA synthesis for in vitro translation
Gene expression studies
mRNA vaccine development and therapeutics
Storage
-20°C for 24 months
Document
The EzRNA™ RNA Capping System is a user-friendly product for post-transcriptional RNA modification. Both Vaccinia Capping Enzyme and 2’-O-Methyltransferase are included in the package, which are able to perform in a single reaction. The Vaccinia Capping Enzyme attach 7-methylguanylate cap (m7Gppp, Cap-0) to the 5′ end of RNA to form m7Gppp5’N-RNA (Cap-0 RNA). The 2′-O-methyltransferase utilizes Cap-0 RNA as a substrate, employing S-adenosine methionine (SAM) as a methyl donor to methylate 2′ -OH of the first nucleotide at the 5′ end of Cap-0 RNA, resulting in the formation of Cap-1 RNA.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main Functions
IVD5412 precast kit for MagMix 32, smart 32
Applications
RT-PCR，PCR，NGS
Products
Viral DNA / RNA, body cell DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technolog
Process method
Manual or automatic
Sample type
Sample amount
200μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
This kit is shipped and stored at room temperature and is valid for 12 months.
Document
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
TL 24 series biological microscope is equipped with 195 achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Details of TL24 series biological microscope
* Optical system: equipped with limited far flat field optical system, complete machine mildew prevention design and large field eyepiece, with beautiful appearance, clear imaging, broad vision, convenient operation and other characteristics, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units
* Observation head device: the cylinder can rotate 360 degrees, the displacement of the center of the mirror is 0.06mm; 30 tilting; the hinge double observation (the left and right systems are 2.0% different; the lens end position is 0.1mm at zero vision) and the third party connection port, pupil distance adjustment range of 55-75mm, fixed observation tube head is internal hexagon screw or select portable screw.
* Eyepiece configuration: random eyepiece WF10X / 18mm; the magnification accuracy of eyepiece shall not exceed ± 2%. There is a card slot on the eyepiece tube to lock and position the eyepiece to prevent falling. Binocular vision is adjustable, making binocular observation easier;
* Loading platform:triangular rail XY composite mechanical moving loading platform; 0.005 lateral to 5N horizontal force, corrosion resistance and wear resistance. Rectangular, size 110X120mm, stroke 7030mm, minimum read value 0.1 mm.
Specification
Specification configuration
TL24A
Binocular observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm
TL24B
Three-eye observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm.
TL24M
Three-eye viewing head, equipped with 5 million pixel imaging system CMOS camera
TL24P
Three-eye viewing head, equipped with 10.1 inch integrated flat panel display
Eyepiece
Wide-angle eyepiece WF10X,field of view 18mm,optional WF16X,WF25X
Converter
Quadruple Revolving Converter
0bjective
195mm achromatic objective,4X,10X,40X,100X(oil)
Condenser
ABBE N.A.1.25Condenser with Iris Diaphragm &Filter
Stage
Double Layer Mechanical Stage,Size110mmx120mm,Moving Range :70mmx30mm
Focusing
Crude motion, micromotion and coaxial focal adjustment system with a minimum focal range of 0.02mm Coarse and Fine Focusing
Light source
1W LED,with Rechargeable Battery,Type C USB cable
Attachments
Dust bag,Type C power cord
Document
TL 24 series biological microscope is equipped with 195 achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
