m-PEG24-DBCO

Features: 

1.Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.

2.Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.

3.Automatic lid lock, LCD display, It can store 100 programs. super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

4.Adapters are available by experiment requirements.

5.3 tiers protection steel cover, safe and reliable. 

6.IEC CE EMC certificate.

 TD5 Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×250ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	570×400×320mm
Net /Gross weight(Kg)	42KG /47KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TD5 

Order No	Rotor	Max	Volume(ml)	Max RCF(×g)
		Speed(r/min)
D5-1	Swing rotor(round	4000	4×250ml	2880
	cup)
D5-2	Swing buckets	4000	4×16×7ml	2860
D5-3		4000	4×16×5ml	2470
D5-4		4000	4×13×10ml	2830
D5-5		5000	4×1×50ml	4730
	Swing rotor
D5-6			4×1×100ml	4730
D5-7			4×2×100ml	3020
D5-8			4×2×50ml	3020
D5-9			4×4×15ml	3020
D5-10			4×6×15ml	3020
D5-11			4×4×10/7	2770
D5-12			4×4×5 vacuum tube	2360
		#######	adapters
D5-13			4×6×10/7	2770
D5-14			4×6×5 vacuum tube	2360
			adapters
D5-15			4×8×10/7	2770
D5-16			4×8×5 vacuum tube	2360
			adapters
D5-17			4×12×5 vacuum tube	2480
D5-18			4×12×7 vacuum tube	2760
D5-19			4×10×10 vacuum tube	2880
D5-20	Microplate rotor	4000	2×3×96 well	2310
D5-21	Fixed rotor	5000	6×15ml	2540
D5-22		5000	12×15ml	3080
D5-23		5000	24×15ml	3500
D5-24		5000	30×15ml	3830
D5-25		5000	4×50ml	2520
D5-26	Fixed rotor	5000	6×50ml	2850
D5-27		4000	12×50ml	2470
D5-28		5000	4×100ml	2630
D5-29		5000	6×100ml	3130

 Adapter for 250 round cup and square cup 

VolumeCup	5ml	7ml	10ml	15ml	50ml
250ml（Square）	9pcs/cup	9	9	4	1
250ml（Round）	7pcs/cup	7	7	4	1

  Rotor with adapter

Introduction

This product is suitable for extracting RNA  from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents. It can specially extract high quality RNA from frozen blood samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents.
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount	1ml whole blood (fresh or frozen blood)

Principles

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR, NGS and virus detection.

Kit Contents: Bottle

Contents	R661301	R661302	R661303
Purification Times	48 Preps	96 Preps	480 preps
DNase I	600 μl	2 x 600 μl	10 x 600 µl
DNase Buffer	20 ml	30 ml	150 ml
MagPure Particles N	2.5 ml	5.0 ml	28 ml
MagZol 3BD	65 ml	140 ml	3 x 200 ml
Buffer ALB2	40 ml	60 ml	350 ml
Buffer BCP2	10 ml	15 ml	80 ml
Buffer MW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	20 ml	60 ml

Storage and Stability

DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N, MagZol 3BD and Buffer BCP2 should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for extracting RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents. It can specially extract high quality RNA from frozen blood samples.

Product Description

Product Detail

Kit Storage and Term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal Nucleic Acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.