m-PEG24-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG24-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG24-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate.
Streptococcus agalactiae TaqMan PCR Kit, 100 reactions
Streptococcus agalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM30650 (100 preps) | Cat. TM30610 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. agalactiae Primer & Probe Mix | 280 μL | 280 μL |
| S. agalactiae Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50mg simple plant using 96 well bind plate |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount | Fresh / frozen plant samples: ≤50mgDried plant / seed samples: ≤15mg |
| Elution volume | ≥75μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316702 | D316703 |
| Purification Times | 4 x 96 Preps | 20 x 96 Preps |
| RNase Solution | 5 ml | 22 ml |
| Elution Buffer | 120 ml | 500 ml |
| Buffer SPL | 200 ml | 2 x 500 ml |
| Buffer PS | 100 ml | 400 ml |
| Buffer GW1 | 220 ml | 5 x 220 ml |
| Buffer GW2 | 100 ml | 3 x 100 ml |
| 1.6ml Collection Plate | 4 | 20 |
| HiPure gDNA Plate | 4 | 20 |
| 2.2ml Collection Plate(DW Plate) | 8 | 40 |
| 0.8ml Collection Plate(DW Plate) | 4 | 20 |
| Sealing Film | 20 | 100 |
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
| HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
Bacillus cereus is a rod-shaped, gram-positive bacterium. Some strains of B. cereus cause foodpoisoning and other diseases such as keratitis. B. cereus grows at a wide range of temperature, from 4°C to 37°C. In fact, many foodborne illnesses caused by B. cereus are a consequence of improperly cooked or improperly stored food, including dairy products and meats. The majority of food poisoning by B. cereus could be divided into two types of symptoms – the emetic type and the diarrheal type, both of which are caused by the enterotoxin produced by the bacteria.
Bacillus cereus TaqMan PCR Kit,
100 reactions
Bacillus cereus TaqMan PCR Probe/Primer Set and Controls,
100 reactions
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM36950 (100 preps) | Cat. TM36910 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Bacillus cereus Primer & Probe Mix | 280 μL | 280 μL |
| Bacillus cereus Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
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