
m-PEG8-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

m-PEG8-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG8-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Figure 1 / 5
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Kit Specifications | |
Column Binding Capacity | 1.5 mg |
Average Yield from 100 mL Culture* | 0.4 – 1.0 mg |
Final Endotoxin Levels | < 0.1 EU/µg DNA |
Time to Complete 4 Purifications | 1.5 hours |
Size of Plasmids Purified | Up to 13,000 bp |
* Depends on high-copy DNA and low copy DNA
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.
Component | Cat. 13300 (50 preps) | Cat. 46400 (250 preps) | Cat. 46500 (4 preps) | Cat. 46600 (20 preps) | Cat. 60300 (50 preps) | Cat. 63000 (192 preps) |
---|---|---|---|---|---|---|
Resuspension Solution AZ | 12 mL | 60 mL | 20 mL | 100 mL | 12 mL | 2 x 20 mL |
Lysis Buffer N | 40 mL | 80 mL | 40 mL | 2 x 80 mL | 40 mL | 2 x 40 mL |
Buffer TN | 20 mL | 130 mL | 55 mL | 2 x 130 mL | 20 mL | 1 x 55 mL 1 x 20 mL |
Wash Solution E | 12 mL | 2 x 18 mL | – | – | – | – |
Elution Buffer K | 8 mL | 30 mL | – | – | 8 mL | 2 x 8 mL |
Wash Solution J | – | – | 25 mL | 3 x 25 mL | – | – |
Elution Buffer J | – | – | 24 mL | 120 mL | – | – |
RNase A | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial |
Magnetic Bead Suspension | – | – | – | – | 1 x 1.1 mL | 4 x 1.1 mL |
Spin Columns | 50 | 250 | – | – | – | – |
Collection Tubes | 50 | 250 | – | – | – | – |
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column) | – | – | 4 | 20 | – | – |
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column) | – | – | 4 | 20 | – | – |
96-Well Plate | – | – | – | – | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – | – | 50 | – |
Elution Tubes (50 mL) | – | – | 4 | 20 | ||
96-Well Elution Plate | – | – | – | – | – | 2 |
Adhesive Tape | – | – | – | – | – | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 |
Organophsphates are a class of pesticides that mechanistically target the acetylcholinesterase enzyme. Regulatory guidelines have been set to ensure our food and water are within the acceptable regulatory authority guidelines. Because most OPs are provided in their precursor form, organothiophosphate (i.e., Malathion, Diazinon, Chlorpyrifos, Azinphos, Dimethoate, Terbufos, Phosmet) Attogene’s organophosphate ELISA kit has been designed to detect organothiophosphates which are the main form of the compounds when applied in the field.
This kit can be used for rapid test of organophosphate in liquid samples such as water, wastewater, and solid samples such as wheat.
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
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