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The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.

• The double-strand specific property of the dsDNase allows decontamination with primers and probe present.
• Efficient for end-point PCR, probe-based qPCR, and some SYBR based qPCR mixes.
• Contaminating bacterial DNA can be reduced to levels below the detection limit.
• Fast and easy protocol.
• Flat NTCs (No Template Controls).

Decontamination of master mixes without reduction of sensitivity has always been a challenge. Especially when minor amounts of DNA are targeted, contaminating DNA is a major problem. Any loss of sensitivity in the qPCR assay caused by the decontamination protocol is unacceptable.

In Figure 1, it is demonstrated that the PCR decontamination kit can remove contaminating DNA from a qPCR mix to non-detectable levels (flat NTC), without affecting the sensitivity of the qPCR.

Kit Contents

• DTT (Inactivation Aid)
• dsDNase

The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 50 & 150 reactions

*Optical system: equipped with infinity plan achromatic stress-free metallographic objective lens,built-in polarizing ober-vation device

*Observation head device:The lens barrel can be rotated 360 degrees,and the interpupillary distance adjustment range is 50-75MM

*Eyepiece configuration:random eyepiece WF10X /22mm;

*Objective:Infinity  plan  achromatic  strain-free   metallographic  objective;5X,10X,20X,50X

*Loading  platform:Stage:Tripod  XY  composite   mechanical  moving  stage; size 140X120MM, stroke 80X50MM, minimum reading value 0.1mm

XTL-20 series metallographic microscopes are equipped with infinity plan achromatic stress-free metallographic objective lens and Wide-angle eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Model:
XTL-20

Brand:
Yingtai