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Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.
Detail
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
The m6A sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Other Products
endo-BCN-PEG3-Gly
Product Info
Document
Product Info
endo-BCN-PEG3-Gly represents an ADC linker featuring a BCN group, a hydrophilic PEG3 linker, and glycine residue. The BCN group is a click chemistry handle that readily reacts with azide groups on a target molecule to form stable triazole linkages. The PEG units enhance the aqueous solubility of the compound and may assist in fine-tuning DMPK properties. This compound may be applied to the synthesis of antibody-drug conjugates.
Document
endo-BCN-PEG3-Gly represents an ADC linker featuring a BCN group, a hydrophilic PEG3 linker, and glycine residue. The BCN group is a click chemistry handle that readily reacts with azide groups on a target molecule to form stable triazole linkages. The PEG units enhance the aqueous solubility of the compound and may assist in fine-tuning DMPK properties. This compound may be applied to the synthesis of antibody-drug conjugates.
EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact cell culture media exosomes for functional studies
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
