Note: Price not include shipment & duty, contact us to get full quote. Magnetic beads are specially designed for nucleic acid extraction and purification, with good suspension performance. The surface is modified with a large number of carboxyl groups, which can bind the nucleic acid in the sample through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acid from biological samples. The operation is safe and simple. It is beneficial to the automation and high throughput extraction of nucleic acid.
Detail
Description
Product introduction
It is suitable for the extraction and purification of viral nucleic acid and free nucleic acid. It can bind the nucleic acids in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acids from biological samples. The operation is safe and simple, which is very conducive to the automatic and high-throughput extraction of short fragments or low abundance nucleic acids.
Product characteristics
Super paramagnetism and high magnetic response, saving operation time and increasing sample recovery efficiency.
Good suspension performance, dispersion and resuspension, which is conducive to efficient nucleic acid binding and recovery.
Good physical and chemical stability to ensure repeatability.
Product information
Other Products
D2111 HiPure Gel DNA Mini Kit
Product Info
Document
Product Info
Introduction
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
Applications
PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Agarose gel, PCR products, enzyme products
Sample amount
Agarose gel: ≤500mg
Recovery
≥80%
Elution volume
≥15μl
Time per run
≤20 minutes（1-24 samples）
Liquid carrying volume per column
800µl
Binding yield of column
35µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – ≥80% DNA recovery
General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
Fast – isolation can be completed in 10-15 minutes by column gel method
Great cost-effectiveness performance
Kit Contents
Contents
D211102
D211103
Purification Times
100 Preps
250 Preps
Buffer GDP
120 ml
250 ml
Buffer DW2
50 ml
2 x 50 ml
Elution Buffer
20 ml
30 ml
HiPure DNA Mini Columns II
100
250
2 ml Collection Tubes
100
250
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data
Document
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Used for the selective isolation and culture of Listeria monocytogenes.
Principle and Interpretation
Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and growth factors，maintains balanced osmotic pressure; Listeria hydrolyzes aesculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Columbia blood agar base
39.0 g
Aesculin
1.0 g
Ferric ammonium citrate
0.5 g
Lithium chloride
15.0 g
pH7.0±0.2 at 25°C
Preparation
Weigh 55.5 g of this product, add 1000 mL of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15 min. Cool to about 50℃, add 1 bottle of SR0500 supporting reagent A and 1 bottle of B per 100 mL, mix well and set aside.
Quality Control
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
Quality control strains
Growth
Listeria monocytogenes ATCC19115
Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212
–
Escherichia coli ATCC25922
–
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use Used for the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and……
Usages: For count, isolation and cultivation of mould and yeast.
Principle: Peptone provides the carbon and nitrogen; glucose to provide energy; potassium dihydrogen phosphate as a buffer; agar as medium coagulant; chloramphenicol inhibit the growth of bacteria; Bengal as selective antibacterial agents inhibit the growth of bacteria.
How to use： 1.Suspend 31.6g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
