Note: Price not include shipment & duty, contact us to get full quote. Magnetic beads are specially designed for nucleic acid extraction and purification, with good suspension performance. The surface is modified with a large number of carboxyl groups, which can bind the nucleic acid in the sample through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acid from biological samples. The operation is safe and simple. It is beneficial to the automation and high throughput extraction of nucleic acid.
Detail
Description
Product introduction
It is suitable for the extraction and purification of viral nucleic acid and free nucleic acid. It can bind the nucleic acids in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acids from biological samples. The operation is safe and simple, which is very conducive to the automatic and high-throughput extraction of short fragments or low abundance nucleic acids.
Product characteristics
Super paramagnetism and high magnetic response, saving operation time and increasing sample recovery efficiency.
Good suspension performance, dispersion and resuspension, which is conducive to efficient nucleic acid binding and recovery.
Good physical and chemical stability to ensure repeatability.
Product information
Other Products
C13113 HiPure cfDNA Column Set
Product Info
Document
Product Info
Introduction
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
C13114 HiPure cfDNA Mini Column Set I (Centrifuge Method)
C13115 HiPure cfDNA Column Set Ⅱ (Vacuum Method)
Details
Specifications
Features
Specifications
Recommended application
Circulating or viral nucleic acid isolation from large volumes of cell free samples (1-5ml)
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/F, 4 layers (3 x GF/F, 1 x GF/B)
Membrane aperture
3 x 0.7μm, 1 x 1.0μm
Maximum binding yield of plasmid
30 μg
Maximum yield of alcohol mediated Binding
200 μg
Single liquid carrying capacity of column
800 μl
Minimum elution volume
30 μl
Withstand centrifugal force
16,000 x g
Centrifuge
Small high speed centrifuge (2ml)
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13113
HiPure cfDNA Mini Column (3 x GF/F, 1 x GF/B)
100/Bag
C13114
HiPure cfDNA Mini Column (3 x GF/F, 1 x GF/B)with 50ml Centrifuge Tube, Extender Tube, Collection Tube, Collection Tube Ⅲ
100/Pack
C13115
HiPure cfDNA Mini Column (3 x GF/F, 1 x GF/B)with Collection Tubes, Extender Tube, Vac-Connector
100/Pack
C13301
Vac connector
100/Bag
C13302
Extender Tube
50/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
SNPsig® VariPLEX SARSCoV-2 is a real-time-reverse transcriptase PCR (RT-PCR) multiplex assay to be used as a reflex qPCR test. VariPLEX™ SARS-CoV-2 can be performed on extracted sample eluates from positive COVID-19 tests.
Detects the variants originally identified in the UK (20I/501Y.V1), South Africa (20H/501Y.V2), Brazil (20J/501Y.V3) and California (20C/S:452R), and the key biologically significant mutations N501Y and E484K, which are now all prevalent globally.
Sputum Liquification Buffer (Cat. 28289) can be purchased separately
This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using spin-column chromatography based on Norgen’s proprietary resin. The kit includes all the reagents needed to process sputum DNA. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.
Background
A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae ;and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.
* Average DNA yield will vary depending on the health status of the donor
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Norgen’s Sputum DNA Isolation Kit contains ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
