Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Our kit detects ssDNA by using fluorescent dye that enables sensitive single stranded DNA quantification , including ssDNA viruses, synthetic ssDNA, first-strand cDNA synthesis, denatured DNA, and bisulfate-converted DNA etc. ssDNA quantification is essential for the study of the biological process involves ssDNA.
Features
A series of input ssDNA (200, 400, 600, 800, 1000, and 1200 ng) was used.
The performance of the BioDynami ssDNA Quantification Kit is nearly identical to that of Thermo Fisher’s Qubit ssDNA kit (figure below).
Comparison of BioDynami ssDNA Quantification Kit with Thermo Fisher kit.
Common contaminants such as salts, solvents, or detergents are well tolerated in the assay (Table 1).
Contaminants has been tested in BioDynami ssDNA Kit.
The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Description
The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.
Spectral Characteristics
When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm. In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.
These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.
Storage
Protected from light
-20°C for 24 months
The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.
Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa. Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.
This kit can be used for quantitative test of Microcystin in liquid samples such as water, wastewater, algal cultures, and solid samples such as fish.
Microcystin-LR
EPA 10-day drinking water health advisories:
Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.05 | 0.1 | 0.2 | 0.4 | 2 ppb
Incubation Time: 75 Minutes
