Mal-amido-PEG4-alkyne

Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.

About

ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.

ProbeSure OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. ProbeSure OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.

Genotype directly from RNA samples. RNA reverse transcription and cDNA PCR genotyping simultaneously in a single, one-step reaction.

Overview

• No need to immediately process samples
• Includes pre-loaded beads for effective sample homogenization by bead beating (tubes are fully compatible with commercially available bead beating systems)
• DNA preservation at room temperature over 2 years
• Ship fecal samples at room temperature safely
• Compatible with most DNA isolation methods 

Norgen’s Fecal DNA Collection & Preservation Mini Tubes are designed for the rapid preservation of DNA from fresh fecal specimens. These tubes contain Norgen’s Fecal DNA Preservative in a liquid format. Moreover, each collection tube is preloaded with beads for effective sample homogenization in any commercially available bead beater equipment (e.g. MP Biomedicals’ FastPrep®-24). The user simply collects fecal samples into the tubes and mixes by shaking or vortexing until the feces is mixed well with preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow the DNA in the samples to be preserved at room temperature for over 2 years. 

This kit is highly suited to collection and preservation of fecal material from mouse, hamster, chinchilla, rabbit and more for microbiome studies and other applications.

DNA Isolation from Preservative

Fecal samples collected and preserved in Norgen’s Fecal DNA Collection and Preservation Mini Tubes are compatible with most fecal DNA isolation methods. Samples stored in the tubes have been used successfully with all of Norgen Biotek’s stool-based DNA isolation kits (please refer to Related Products table) and reagents.  

Free Download

Extracting Biological Insights from Stool

Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free

Details

Supporting Data

Figure 1 / 4

Previous

Next

Click for expanded view

Kit Specifications
Fecal Input	Maximum 30 mg (approximately 3-5 mouse droppings)
Stability of Fecal DNA at Room Temperature	2 years

Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Kit Components	Cat. 27650
Fecal DNA Collection & Preservation Mini Tubes (containing liquid preservative and beads)	12
Product Insert	1