Introduction

MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Details

Specifications

Features	Specifications
Main Functions	Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure)
Applications	Sequencing, gene chip and qPCR, etc.
Purification technology	Magnetic beads technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR products, enzymatic reaction solution
Sample amount	Appropriate
Elution volume	≥15μl
Operation time	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excessprimes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.

Advantages

• Wide application – DNA products, enzyme digestion system, PCR reaction
• High recovery – suitable for 100bp-30kb fragments
• High purity – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
• Strong applicability – manual operation or automatic workstation

Kit Contents

Contents	BXP-5	BXP-50	BXP-500
MagPure A3	5 ml	50 ml	500 ml

Storage and Stability

MagPure A3 should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.DO NOT FREEZE.

MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Overview

• Purify amplified DNA ranging from 100 bp -15,000 bp in size
• Fast and efficient spin column format 
• Available in a 50 prep size and a 250 prep size
• Also available in 96 well format
   

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

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Kit Specifications – Spin Column
Column Binding Capacity	10 μg
Size of DNA Purified	100 – 15,000 bp
Average Recovery	> 90%
Average Primer Removal	> 90%
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	15 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 14400 (50 preps)	Cat. 45700 (250 preps)	Cat. 24800 (192 preps)
Binding Buffer C	30 mL	5 x 30 mL	3 x 30 mL
Wash Solution A	12 mL	2 x 20 mL	2 x 38 mL
Elution Buffer B	8 mL	2 x 30 mL	2 x 15 mL
Spin Columns	50	250	–
Collection Tubes	50	250	–
96-Well Plate	–	–	2
Adhesive Tape	–	–	4
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	250	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	2

Overview

• High quality DNA, free from RNA contamination
• Isolate genomic DNA from anticoagulated and untreated blood
• Rapid and convenient spin column procedure
• Isolate DNA from inputs as low as 20 µL

This kit is designed for the rapid preparation of total DNA from dried blood spots with a quick and convenient spin column.  The Dried Blood Spot DNA Isolation Kit allows for the isolation of DNA from the blood of various species, including humans. Preparation time for a single sample is 30 minutes.  The purified DNA is of high quality and is completely compatible with downstream applications including PCR, qPCR and more.

Details

Supporting Data

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Kit Specifications
Maximum Blood Input	3 x 3 mm diameter punches
Average yield* (ng)	150 ng
Column Binding Capacity	> 50 µg
Time to Complete 10 Purifications	35 minutes

 * Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 36000 (50 preps)
Digestion Buffer B	6 mL
Lysis Buffer B	20 mL
Wash Solution WN	18 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	1.2 mL
Micro Spin Columns	50
Collection Tubes	50
Elution Tubes	50
Product Insert	1