Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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COVID-19 Real-Time PCR assay EUA (USA)
Product Info
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Product Info
This test has been authorized by FDA under an EUA for use by authorized laboratories.
The Primerdesign Ltd COVID-19 genesig® Real-Time PCR assay is a real-time RT-PCR assay intended for the qualitative detection of nucleic acid from SARS-CoV-2 in oropharyngeal swab specimens from patients suspected of COVID-19 by their healthcare provider. Testing is limited to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, to perform high complexity tests.
FOR US ONLY Rapid detection and exclusive to the COVID-19 strain Does not detect other related coronavirus strains High priming efficiency Accurate controls to confirm extraction, and assay validity Lyophilised components for ambient shipping Highly specific detection profile This test has been authorized by FDA under an EUA for use by authorized laboratories.
Propargyl-PEG7-NHS ester contains a propargyl group and an NHS ester. As an amine reactive reagent, this product can be used for derivatizing peptides, antibodies, amine coated surfaces etc. In the presence of copper catalyst, propargyl group reacts with azide to yield a stable triazole linkage. The PEG units help increase the hydrophilicity of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG7-NHS ester contains a propargyl group and an NHS ester. As an amine reactive reagent, this product can be used for derivatizing peptides, antibodies, amine coated surfaces etc. In the presence of copper catalyst, propargyl group reacts with azide to yield a stable triazole linkage. The PEG units help increase the hydrophilicity of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The NGS Single Stranded DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using sheared single stranded DNA (50 ng – 500 ng) as input. The kit is ideal for making NGS libraries with samples of denatured DNA, viral DNA, highly degraded ancient DNA and other single stranded DNA. The workflow of the ssDNA kit is simple: make the libraries in two steps followed by PCR and cleanup steps. The libraries will be ready in just 1.5 hours with a 10 minutes of hands-on time. The library pooling is possible dependent on the type of index. The final library is strand specific.
NGS Single Stranded DNA Library Prep Kit workflow
Three index types are available for the kit:
Non-index (Cat.# 30081): Libraries do not have index.
Index(Cat.# 30082): Each of the index primers has a unique 6-base index sequence that can be used to identify libraries. ssDNA library multiplexing is up to 48 samples. Index information can be downloaded here.
Unique dual index (Cat.# 30083): ssDNA sample multiplexing up to 96 libraries is possible with unique dual indexes. We have developed a Four-Base Difference Index System. This makes it possible to generate indexes with at least 4 bases different from each other in the 8 bases index length. The unique dual indexing primers remove NGS errors (example: de-multiplexing errors, read mis-assignment, index hopping etc). Index information can be downloaded here.
Kit advantages:
Quick and simple Protocol
Quick: Total protocol time is only 1.5 hours
Simple: Hands-on time only 10 minutes
Easy procedure based on:
The ready-to-use master mix: Made reaction setup easy
Less reaction components: Simplify the reaction preparation
Less magnetic beads needed: Reduced more than 50% of the beads for cleanup steps
Directional library
Single stranded DNA as input: From 50 ng to 500 ng
NGS Single Stranded DNA Library Prep Kit has similar library conversion efficiency and yield as compared to a regular DNA library prep kit.
Alignment rate and duplication rate: comparison of single stranded DNA library kit versus double stranded DNA library prep kit.
Document
The NGS Single Stranded DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using sheared single stranded DNA (50 ng – 500 ng) as input. The kit is ideal for making NGS libraries with samples of denatured DNA, viral DNA, highly degraded ancient DNA and other single stranded DNA. The workflow of the ssDNA kit is simple: make the libraries in two steps followed by PCR and cleanup steps. The libraries will be ready in just 1.5 hours with a 10 minutes of hands-on time. The library pooling is possible dependent on the type of index. The final library is strand specific.