Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
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| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Maximum Blood Input* | 1 mL or 1.5 x 106 Leukocytes |
| Standard Blood Input | 150 μL |
| Minimum Blood Input | 10 μL |
| Average Yield: 500 μL human blood | 1.5 μg |
| Time to Complete 10 Purification | 40 minutes |
*Additional RBC Lysis Solution is required for input Volumes >150 µL
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 21200 (50 preps) | Cat. 21250 (50 preps) | Cat. 37800 (2 x 96 preps) |
|---|---|---|---|
| RBC Lysis Buffer | 2 x 100 mL | 2 x 1 L | 2 x 90 mL |
| Buffer RL | 30 mL | 30 mL | – |
| Binding Solution | – | – | 2 x 40 mL |
| Wash Solution A | 38 mL | 38 mL | – |
| Wash Solution | – | – | 2 x 30 mL |
| Elution Solution A | 6 mL | 6 mL | – |
| Elution Solution | – | – | 2 x 20 mL |
| Enzyme Incubation Buffer | – | 6 mL | 1 |
| DNase I | – | 1 Vial | – |
| Mini Spin Columns | 50 | – | – |
| Lysate Homogenization Column | – | 50 | – |
| Single Cell RNA Column | – | 50 | – |
| RBC Lysis 96-Well Plate | – | – | 2 |
| 96-Well Filter Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Collection Tubes | 50 | 100 | – |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 50 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 1 |
What is the genesig q16?
The genesig q16 is a revolutionary instrument launched by Primerdesign Ltd. The instrument is designed to accompany the genesig Easy product range which includes kits for more than 550 different DNA testing applications. The genesig q16 is designed to make DNA testing affordable and easy for anyone in any business.
What is DNA testing?
DNA testing is the most sensitive and precise way to detect and quantify the presence of a DNA target. The underlying technology within the genesig q16 is real-time quantitative PCR. The technology has been around for 20 years, but to date has been complex and expensive to perform. The genesig q16 changes all that.
What can I use it for?
The genesig Easy product range includes tests for a massive range of applications:
Incredibly easy to use
Automated data analysis
Robust, beautiful design
Small and portable: 12cm footprint
Over 550 kits available from a vast range of human, food and veterinary detection profiles
Highly portable
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
