Methyltetrazine-PEG23-DBCO

Features: 

1. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running

2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of  high quality steel, safe and reliable.

3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.

4. Microprocessor control.

5. Digital display in time, speed and RCF.

6. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

7. 3 tiers protection steel cover, stainless steel chamber, safe and reliable.

 TG20Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	6×100ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤60
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	513×370×320mm
Net Weight/Gross weight(Kg)	43KG/60KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TG20 

Order no	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(*g)
G20-1	Fixed Rotor	16000	4×8PCR	15760
G20-2	Fixed Rotor	15000	6×8PCR	21420
G20-3	Fixed Rotor	16000	8×8PCR	17480
G20-4	Fixed Rotor	15000	12×8PCR	22930
G20-5	Fixed Rotor	21000	12×1.5/2ml	30910
G20-6	Fixed Rotor	15000	40×0.5ml	22920
G20-7	Fixed Rotor	17000	24×1.5/2ml	26460
G20-8	Fixed Rotor	13500	30×1.5/2ml	19340
G20-9	Fixed Rotor	13000	48×1.5/2ml	17930
G20-10	Fixed Rotor	16000	16×5ml	22020
G20-11	Fixed Rotor	16000	6×10ml	21500
G20-12	Fixed Rotor	15000	12×10ml	22680
G20-13	Fixed Rotor	16000	12×7ml	21380
G20-14	Fixed Rotor	13000	16×10ml	19490
G20-15	Fixed Rotor	11000	12×15ml	14330
G20-16	Fixed Rotor	13000	8×15ml(conical)	17790
G20-17	Fixed Rotor	5000	24×15ml	3500
G20-18	Fixed Rotor	15000	8×20ml	22680
G20-19	Fixed Rotor	5000	30×15ml	3830
G20-20	Fixed Rotor	14000	6×30ml	19060
G20-21	Fixed Rotor	13000	6×50ml(conical)	18840
G20-22	Fixed Rotor	13000	6×50ml(round)	18730
G20-23	Fixed Rotor	13000	4×85ml	18940
G20-24	Fixed Rotor	12000	6×70ml	15570
G20-25	Fixed Rotor	12000	4×100ml	14850
G20-26	Fixed Rotor	10000	6×100ml	11380
G20-27	Fixed Rotor	18000	30×0.5ml	26660
G20-28	Swing Rotor	13000	4×5ml	14960
G20-29	Vertical Rotor	16000	16×5ml	16540
G20-30	Vertical Rotor	14000	8×30ml	16450
G20-31	Microplate Rotor	4000	2×3×48（well）	2300

OverView

HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human  total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results  indicate that HL-dsDNase has minimal impact on RNA quality and quantity.

HL-dsDNase is an engineered version of dsDNase that is rapidly and completely inactivated by incubation for 5 minutes at 58°C with 1 mM DTT at pH 8.0 or above. Chemical inactivation in downstream compatible RT or PCR buffers allows milder heat inactivation and, in some cases, skipping heat inactivation altogether. This makes HL-dsDNase very useful for removal of DNA from RNA preparations since the enzyme may be inactivated using various strategies with reduced risk of auto-degradation of  RNA in the presence of magnesium, making HL-dsDNase an ideal enzyme when working with small volumens of RNA.

HL-dsDNase is also an excellent choice for removal of unwanted external DNA from samples prior to analysis of nucleic acids protected by biological membranes, e.g., bacteria, viruses, and sperm. Especially if using downstream analysis methods that might be affected by host cell DNA, as metagenomic sequencing and STR-profiling. The easy inactivation of HL-dsDNase makes it a fast and efficient alternative to methods as differential extraction, where sample material is often lost.

Recommended applications:

• Removal of genomic DNA from RNA preparations
• Improved removal of host-cell DNA from sperm cells prior to STR-profiling

Key advantages with HL-dsDNase:

• Double-strand DNA specific endonuclease
• Easily heat-inactivated by very moderate heat treatment
• High specific activity
• Useful for removal of DNA from RNA preps

Properties

HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human  total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results  indicate that HL-dsDNase has minimal impact on RNA quality and quantity.

Introduction

This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from tissue, cell, whole blood
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Tissues, cells, lymphocytes and other clinical sample
Sample amount	Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture

Kit Contents

Contents	IVD3020B-96	IVD3020B
Purification Times	96 Preps	200 Preps
MagPure Particles N	2.5 ml	5 ml
DNase I	2 x 600 µl	4 x 600 µl
DNase Buffer C	60 ml	120 ml
Buffer RLC	60 ml	120 ml
Buffer MCB*	60 ml	150 ml
Buffer GW1*	44 ml	110 ml
Buffer MW2*	50 ml	100 ml
RNase Free Water	20 ml	60 ml

Storage and Stability

DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.

This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.