Methyltetrazine-PEG23-DBCO

Description

Ractopamine ELISA Kit

Ractopamine ELISA Test Kit is a competitive enzyme immunoassay for detection in meat, feed, liver, kidney, milk, urine, serum and plasma. Ractopamine has been banned for use as a livestock growth promoter in many countries around the world. To prevent ractopamine residues from entering the food chain, both producers and government surveillance agencies need technologies and methods that can provide rapid, accurate and reliable detection at specific sensitivities. This kit enables government agencies, food manufacturers, as well as quality assurance organizations, to detect ractopamine as low as 0.1 ng/g or ppb level in a variety of sample types.

The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.

NGS FFPE DNA Library Prep Kit Workflow

FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples.  Thus, the extraction of high quality DNA from FFPE samples is often a challenge.  Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation.

As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples.

Three index types are available for the NGS FFPE DNA Library Prep Kit of the illumina platform:

Non-index (Cat.# 30035): Libraries do not have index.

Index (Cat.# 30037): Each of our index primers contains a unique barcode DNA (6 bases long) that can be used to identify individual library. Multiplexing of libraries is up to 48 samples. Index information can be downloaded here.

Unique dual index (Cat.# 30039): FFPE DNA library multiplexing is possible with 96 samples based on the unique dual indexing system. Our unique Four–Base Difference Index System allows us to make indexes that have at least 4 bases different from each other in the 8-base index sequence. Our unique dual indexing primers can effectively remove NGS errors including index hopping, de-multiplexing errors, index cross-contamination, mis-assignments etc. The unique dual index primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34037).

Kit advantages:

• Fast and simple protocol
  • Making libraries in just 1.5 hours
  • 10 minutes of hands-on time
• Easy procedure
  • Ready-to-use master mix to reduce the tedious mixing
  • Less reaction components to simplify the reaction setup
• Less magnetic beads needed for the purification steps: saving more than 50% of the expensive beads
• Low input FFPE DNA: From 10 ng to 400 ng

Comparison of library conversion efficiency under the same condition. Input DNA amount is 25 ng.

Comparison of library yield under the same condition. Input DNA amount is 25 ng.

The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.

The Opentrons Temperature Module, compatible with both the OT-2 and Flex Robots, is a fully automated thermoelectric module and integrated for use with the Opentrons platform. It is used to achieve temperature changes ranging from 4 to 95°C for various applications.

The Temperature Module GEN2 is compatible with the new Opentrons Flex Robot when paired with the Flex Temperature Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.

The Opentrons Temperature Module, compatible with both the OT-2 and Flex Robots, is a fully automated thermoelectric module and integrated for use with the Opentrons platform. It is used to achieve temperature changes ranging from 4 to 95°C for various applications.

The Temperature Module GEN2 is compatible with the new Opentrons Flex Robot when paired with the Flex Temperature Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.