Methyltetrazine-PEG23-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG23-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The kit includes HS Dye, HS Dilution Buffer, and two DNA Standards. The assay is accurate for DNA concentrations from 10 pg/µL to 100 ng/µL, and is highly selective for double-stranded DNA over RNA.
Features
DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.
Comparison of dsDNA HS kits. The performance of the BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.
Cat.# 40042 dsDNA Quantification HS Kit, 100 assays and 500 assays
Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.
Vibrio cholerae TaqMan PCR Kit, 100 reactions
Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM38550 (100 preps) | Cat. TM38510 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| V. cholerae Primer & Probe Mix | 280 μL | 280 μL |
| V. cholerae Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Protease K is a serine protease with high enzymatic activity and wide substrate specificity. It can preferentially decompose ester and peptide bonds adjacent to the ends of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids C, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and two Ca2+ binding sites around the active center increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.
Appearance: Colorless to light brown liquid; Specific activity: ≥800 U/mL; Protein concentration: ≥20 mg/mL
Source: Tritirachiumalbum; Category: EC 3.4.21.64; Molecular weight: 29 kDa(SDS-PAGE); Isoelectric point: 7.81; The optimal pH: 7.0-12.0 has high activity; Optimum temperature: 65℃; pH stability: pH 4.5-12.5(25℃,16 h); Thermal stability: stable below 50℃ (pH 8.0,30 min); Storage stability: 25℃ a year activity of more than 90% activator SDS, urea; Inhibitors: diisopropyl fluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: 2-8℃, valid for 24 months.
Wear protective gloves and goggles when using, and keep well ventilated after use. This product may cause allergic skin reactions.
Note: Price not include shipment & duty, contact us to get full quote.
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