Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG4-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.
Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.
Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM37150 (100 preps) | Cat. TM37110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Clostridium difficile Primer & Probe Mix | 280 μL | 280 μL |
| Clostridium difficile Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Specifications
| Features | Specifications |
| Main FunctionsC | Co-isolation total RNA and DNA from FFPE tissue |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Sample type | FFPE slice, FFPE puncture sample, embedded tissue |
| Sample amount | No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area. |
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
| Contents | IVD3026 |
| Purification Times | 200 Preps |
| MagBind Particles | 9.0 ml |
| Proteinase K | 180 mg |
| Protease Dissolve Buffer | 10 ml |
| Buffer DPS | 150 ml |
| Buffer FRL | 40 ml |
| Buffer ATL | 40 ml |
| Buffer AL | 80 ml |
| Buffer BXW1* | 110 ml |
| RNase Free Water | 30 ml |
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
