Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
LowRanger 100 bp DNA Ladder
Product Info
Document
Product Info
Overview
Ready-to-use
Quantitative
Highly Stable
Precise
Eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
Higher intensity reference band at 500 bp
The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for fast running times and accurate visual determination.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
LowRanger 100bp DNA Ladder (Cat# 11500) – 100 loads
Ladder Properties: • Eleven discrete bands, ranging from 100 bp to 2000 bp • Higher intensity band at 500 bp for easy reference.
Fragment
Size (bp)
Mass (ng)
1
2000
110
2
1500
83
3
1000
55
4
800
44
5
700
39
6
600
33
7
500
55
8
400
22
9
300
17
10
200
22
11
100
22
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
CE-IVD marked in accordance with EU Directive 98/79/EC
Ideal for use in in vitro diagnostic workflows
Fixative-free preservative, no cross-linking of DNA
Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C.
Preserve cf-RNA for 30 days at ambient temperature
Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
No plasma volume loss after shipping/transportation
Prevent hemolysis allowing better separation of plasma
Prevent apoptosis of blood cells and fragmentation of genomic DNA
Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
Vacuumed to draw 8.4 mL of blood in 10 mL tubes
The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume.
These tubes are ideal for collecting and preserving cf-DNA and cf-RNA samples for in vitro diagnostic use for medical purposes.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes Dx preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C.
Workflow
NOTE: This product is not available for sale in the United States. For the research use only version, see Cat. 63950
Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C
Length of cf-RNA Preservation
Up to 30 days at room temperature (15-25°C)
Length of CTCs Preservation
Up to 14 days at room temperature (15-25°C)
Storage Conditions and Product Stability
When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx are stable through the expiration date. See tube label for expiry date.
Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx.
Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 8 days when stored at 37ºC.
Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are stable for up to 14 days at room temperature (15-25ºC).
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
α-Amylase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.003 U/g
Reproducibility (%):
~ 3%
Total Assay Time:
~ 30 min
Application examples:
Cereal flours, fermentation broths and other materials.
Method recognition:
AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018)
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
All reagents stable for > 2 years after preparation
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Validation of Methods
Document
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).