Ten discrete fragments ranging from 300 bp to 5000 bp
The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.
Contents: 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Ladder Properties: • Ten discrete fragments ranging from 300 bp to 5000 bp
Fragment
Size (bp)
Mass (ng)
1
5000
88
2
4000
78
3
3000
67
4
2500
58
5
2000
50
6
1500
43
7
1000
25
8
700
34
9
500
33
10
300
24
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Other Products
D3115 HiPure Tissue&Blood DNA Maxi Kit
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 10ml blood and 1g tissue using Maxi column
Applications
PCR, southern bolt and virus detection, etc
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount
3-10ml
Elution volume
≥700μl
Time per run
≤90 minutes
Liquid carrying volume per column
4ml
Binding yield of column
5mg
Principle
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
Wide applicability – handle a variety of liquid samples
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction
Select the right purification kit to get impactful results：
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications
qPCR, NGS, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Serum, plasma
Sample amount
0.2-0.6ml
Elution volume
≥30μl
Time per run
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
Economy – less than 50% of the price of Qiagen and other imported products
Automatic – without labour
Kit Contents
Contents
IVD5432
Purification Times
200
MagPure Particles G
14 ml
Carrier RNA
310 μg
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer MLK
250 ml
Buffer MAW1
250 ml
Buffer MW2*
2 x 50 ml
Elution Buffer
60 ml
Contents
IVD5432-F-96A
IVD5432-F-96B
IVD5432-F-96C
Sample amount
200～350μl
400~700μl
Carrier RNA
110 μg
110 μg
Proteinase K
50 mg
100 mg
Protease Dissolve Buffer
5 ml
6 ml
Elution Buffer
15 ml
15 ml
Tip
1
1
Sample Plate A
500μl Buffer MLK
500μl Buffer MLK
Sample Plate B
/
500μl Buffer MLK
Wash Plate 1
700μl Buffer MAW1
700μl Buffer MAW1
Wash Plate 2
25μl Buffer MPG2700μl Buffer MW2
25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3
700μl Buffer MW2
700μl Buffer MW2
Elution Plate
/
/
Contents
IVD5432-TL-06A
IVD5432-TL-06B
IVD5432-TL-06C
Sample amount
300~350μl
600~700μl
900~1050μL
Carrier RNA
310 μg
310 μg
310 μg
Proteinase K
50 mg
100 mg
150 mg
Protease Dissolve Buffer
6 ml
6 ml
10 ml
Elution Buffer
15 ml
15 ml
15 ml
DA-Tip
12
12
12
Row 1/7
600μl Buffer MLK
600μl Buffer MLK
600μl Buffer MLK
Row 2/8
/
600μl Buffer MLK
600μl Buffer MLK
Row 3/9
600μl Buffer MAW1
600μl Buffer MAW1
600μl Buffer MLK
Row 4/10
20μl Buffer MPG2600μl Buffer MW2
20μl Buffer MPG2600μl Buffer MW2
30μl Buffer MPG2900μl Buffer MAW1
Row 5/11
600μl Buffer MW2
600μl Buffer MW2
900μl Buffer MW2
Row 6/12
/
/
/
Storage and Stability
Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.
Document
MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
