Milenia GenLine Extraction System

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Purification and enrichment of intact cell culture media exosomes for functional studies
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents nor overnight incubation required
• Pure exosomes are purified and are free-from any other RNA-binding proteins
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	20 ml to 35 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40-45 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

This H14 filter is a replacement for use with the OT-2 HEPA Module GEN1. The HEPA Module enables you to run sensitive contamination-prone applications on the OT-2.

This H14 filter is a replacement for use with the OT-2 HEPA Module GEN1. The HEPA Module enables you to run sensitive contamination-prone applications on the OT-2.

 Features: 

1.Brushless frequency motor,in great torque, free maintenance, no powder pollution, quick in speed up and down. 

2.Imported compressors fluorine free,double cycle cooling,cold and hot alternating easily, free environment pollution and precise in temperature control. 

3. Microprocessor control, digital display which indicates the speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.

6. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.

7. There are several kinds of rotors for your choice, adapters can be designed by experimental requirements.

8. 3 tiers protection steel cover and get the ideal centrifugation result.

 TDL5M Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×800ml( 4*450ml blood bags)
Time Range	0~99h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20℃-40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	700×715×420mm
Net Weight(Kg)	125KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TDL5M 

Order No.	Rotor	Max Speed (rpm)	Max Volume(ml)	Max. RCF（×g）
NO30671	Swing Rotor	4000	4×800ml(Round Cup)	3450
NO30696	Swing Rotor	4000	4×500ml( Round Cup)	3380
NO30679	Swing Rotor	4000	4×500ml(Square Cup)	3310
NO31494	Microplate Rotor	4000	4×4×96 Well	2840
NO31491		4000	2×4×96 Well	2490
NO31376		4000	2×3×48 Well	2300
NO30690	Swing Rotor	4000	4×30×7ml	3270
			4×30×5ml	2890
			4×18×10ml	3140
NO30607	Angle Rotor	5000	12×15ml	3080
NO30611	Angle Rotor	5000	6×50ml	2850
NO30640	Angle Rotor	5000	4×50ml	2520
NO30613	Angle Rotor	5000	4×100ml	2630
NO30639	Angle Rotor	5000	24×15ml	3500
NO30641	Angle Rotor	5000	12×50ml	3860
NO30614	Angle Rotor	5000	6×100ml	3130
NO30642	Angle Rotor	4000	12×100ml	2970

 Adapter for 800ml and 500ml bucket 

VolumeCup	5ml	7ml	10ml	15ml	50ml
800ml（Round Cup）	23pcs/cup	23	19	14	5
500ml（Round Cup）	18pcs/cup	18	14	9	3
500ml（Square Cup）	20pcs/cup	20	16	12	5