Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.
Detail
Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.
Other Products
IVD3018 HiPure Universal DNA Kit
Product Info
Document
Product Info
Introduction
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications
PCR, qPCR, southern bolt and virus detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Wide applicability – It can handle various liquid samples, animal tissues and cultured cells
Kit Contents
Contents
IVD3018
Purification Times
100
HiPure DNA Mini Columns I
100
2ml Collection Tubes
2 x 100
Buffer ATL
60 ml
Buffer AL
60 ml
Buffer GW1
44 ml
Buffer GW2
50 ml
Proteinase K
60 mg
Protease Dissolve Buffer
5 ml
Buffer AE
15 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Content:
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Acetic Acid
Assay Format:
Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
up to 1.8 g/L of acetic acid per assay
Limit of Detection:
~ 10 mg/L
Reaction Time (min):
8 min at 25oC or 5 min at 37oC
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Improved method
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
As part of Megazyme’s overall commitment to providing the highest quality products, we have developed this acetic acid kit that provides a specific and rapid assay for use with auto-analysers. The kit assay is based on the conversion of NAD+ to NADH and therefore provides a positive reaction (increase in absorbance) which offers a more robust assay.
The reagents, as supplied, are stable for a minimum of 2 years and the prepared reagents are stable for a minimum of 1 week (on-board stability). In addition, the prepared reagents can be stored frozen for longer term stability (see K-ACETGK Booklet for more details).
View more of our acetic acid and organic acid assay kits.
Advantages
Excellent reagent stability
> 7 days at 4oC or > 2 years below -10oC when prepared for auto-analyser applications
> 2 years as supplied
Very rapid reaction (~ 5 min at 37oC)
Linear calibration (R2 ~ 0.997 up to 1.8 g/L sample)
Document
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
DBCO-PEG4-PC represents a photocleavable, PEG linker consisting of a DBCO group that can be used for copper-free Click Chemistry reactions. This structure allows for efficient photorelease of linked molecules, typically achieving over 90% cleavage in 5-25 minutes using a near-UV lamp. The PEG4 spacer increases the hydrophilicity of the compound.
Document
DBCO-PEG4-PC represents a photocleavable, PEG linker consisting of a DBCO group that can be used for copper-free Click Chemistry reactions. This structure allows for efficient photorelease of linked molecules, typically achieving over 90% cleavage in 5-25 minutes using a near-UV lamp. The PEG4 spacer increases the hydrophilicity of the compound.