Introduction

This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.

Details

Specifications

Features	Specifications
Main Functions	Extract pathogen DNA/RNA from whole blood, body fluid, serum, plasma, sputum, immersion solution, tissue homogenate supernatant, etc.
Applications	RT-PCR，PCR，NGS
Products	Pathogen DNA and RNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Whole blood, plasma, serum, immersion solution, tissue homogenate supernatant
Sample amount	200μl
Elution volume	≥30μl
Time per run	30 mins
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA is finally eluted with low-salt buffer (10 MmTris, pH 8.0).

Advantages

• Fast – column purification, several samples can be finished in 30 mins
• High quality – high purity total RNA/DNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

Kit Contents

Contents	IVD4179
Purification Times	50 Preps
HiPure Viral Mini Column	50
2ml Collection Tubes	100
2ml Bead Tubes	50
Protease K	50 mg
Protease Dissolve Buffer	5 ml
DNase I (Powder)	10 mg
DNase Buffer	6 ml
Buffer CLB	100 ml
Buffer SDS	5 ml
Reagent DX	1.5 ml
Buffer ACL	30 ml
Buffer VHB*	22 ml
Buffer RW2*	20 ml
Buffer AVE	15 ml

Storage and Stability

Proteinase K and DNase I (Powder) should be stored at 2–8°C upon arrival. However, short-term storage (DNase I up to 1 week, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.

Introduction

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
Applications	Second generation sequencing, PCR, real timePCR, etc.
Purification method	Monodisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount	Body fluid : 10 – 200μl, Tissue : ≤10mg
Yield	10ng – 15μg
Elution volume	80 -100μl
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
• High purity – carboxyl magnetic beads with weak surface adsorption, getting higher purity
• General – can be used for various clinical samples

Kit Contents

Contents	IVD3101
Purification Times	200
MagBind Particles	4.5 ml
Proteinase K	90 mg
Protease Dissolve Buffer	10 ml
Buffer ATL	60 ml
Buffer AL	60 ml
Buffer BD*	20 ml
Buffer BXW1*	110 ml
Elution Buffer	30 ml

Storage and Stability

Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Introduction

Intended Use

For the selective separation and enumeration of enterococci in food and water.

Principle and Interpretation

Tryptone and peptone are the sources of nitrogen and essential growth factors. Yeast extract acts as well nitrogenous compounds and additionally the vitamin B12 complex. Sodium azide acts largely inhibits the growth of gram-negative bacteria while sparing enterococci, staphylococci and streptococci. Ox bile inhibits most gram positives but not enterococci. Enterococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing a brownish black precipitate around the colonies. Tolerance to bile and the ability to hydrolyze esculin is the traditional and reliable test for the identification of enterococci. (4). Sodium chloride maintains the osmotic balance of the medium and Agar is the solidifying agent.

Formulation

Ingredients	/liter
Tryptone	17.0g
Ox bile	10.0g
Yeast extract	5.0g
Sodium chloride	5.0g
Peptone	3.0g
aesculin	1.0g
Ferric ammonium citrate	0.5g
Sodium azide	0.15g
Agar	15.0g
pH 7.1±0.1 at 25°C

Preparation

Weigh 56.6g of dry powder of this product, add 1 L of distilled water or deionized water, stir, heat and boil until completely dissolved, and sterilize at 121℃ for 15 min.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 20-24hours.

Quality control strains	Growth	Colony color
Enterococcus faecalis ATCC29212	PR≥0.7	Brown-black halo
Escherichia coli ATCC25922	inhibited	Absence of brown-black halo

Sorage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the selective separation and enumeration of enterococci in food and water. Principle and Interpretation Tryptone and peptone are the sources of nitrogen and essential grow……