Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
RNA/DNA/Protein Purification Plus Kits
Product Info
Document
Product Info
Overview
Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
No sample splitting or need to use phenol or precipitation methods
Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Maximum Amount of Starting Material: Animal Cells Animal Tissues Blood Bacteria Yeast Fungi Plant Tissues
5 x 106 cells 25 mg (for selected tissues) 100 μL 1 x 109 cells 1 x 108 cells 50 mg 50 mg
Time to Complete 10 Purifications
30 minutes
Average Yield:HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)
15 μg RNA8 μg DNA150 μg protein
Storage Conditions and Product Stability The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Key features：
1. High-definition LCD touch screen, simultaneously displaying operational parameters: speed, temperature, vacuum, and running time.
2. Delayed start: after reaching a fixed speed, Start the vacuum pump to effectively prevent sample mixing.
3. Fully automatic vacuum control system, vacuum setting range0.1~1000mbar, Control accuracy0.1mbar.
4. 1.5/2ml Rotor stacking for simultaneous use; rotor replacement without tools.
5. Programmable with temperature and pressure settings; capable of storing 36 programs
6. Compatible with various rotor heads; transparent glass window for easy observation.
7. Magnetic drive: maintenance-free, non-contact drive, fully sealed.
8. Safety in use: inductive electric self-suction door lock ensures the centrifugal concentrator can only start when the door cover is fully closed and locked.
9. Can be used in conjunction with freeze-drying systems
Model:
SD-25/SD-25C
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
α-Amylase, β-Amylase
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.05 U/mL
Reaction Time (min):
~ 20 min (Ceralpha Method), ~ 10 min (Betamyl-3 Method)
Application examples:
Cereal flours, malts, fermentation broths and other materials.
Method recognition:
“Ceralpha” Method: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018). “Betamyl-3” Method: RACI Standard Method
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
