Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
Fecal Swab Collection and Preservation System
Product Info
Document
Product Info
Overview
No need to immediately process samples
Nucleic acid preservation at room temperature over 2 years for DNA and up to 7 days for RNA
Norgen’s Fecal Swab Collection and Preservation System is designed for collection, ambient storage and transport of nucleic acids from stool specimens. The Fecal Swab Collection and Preservation System tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Stool Preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended upon arrival at the testing facilities until RNA purification.
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Extracting Biological Insights from Stool
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
Stability of Stool Nucleic Acids at Room Temperature
2 years for DNA Up to 7 days for RNA*
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
The Fecal Swab Collection and Preservation System (Bulk Format) can be stored at room temperature for up to 2 years without any reduction in kit performance. The expiry date for the swabs is written on the swab wrapper.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main Functions
IVD5412 precast kit for kingfisher Flex
Applications
RT-PCR，PCR，NGS
Products
Viral DNA / RNA, body cell DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technolog
Process method
Manual or automatic
Sample type
Sample amount
200μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
Cat.No
Reagent
IVD5412-F-96
PK/Carrier RNA
1x 24 mg/Bottle
Protease Dissolve Buffer Blue
1.8 ml/Bottle
Tip
1
Sample Plate (DW Plate)
500µl Buffer MLB/Well
1
Wash 1 Plate (DW Plate)
500µl Buffer MW1/Well
1
Wash 2 Plate (DW Plate)
500µl Buffer CW/Well
1
Beads Plate (DW Plate)
500µl CW & 20µl MPN/Well
1
Elute Plate (KF Plate)
90µl NFW/Well
1
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
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This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Purify amplified DNA ranging from 100 bp -15,000 bp in size
Fast and efficient spin column format
Available in a 50 prep size and a 250 prep size
Also available in 96 well format
This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
