Introduction

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	0.2-0.6ml
Elution volume	≥30μl
Time per run	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour

Kit Contents

Contents	IVD5432
Purification Times	200
MagPure Particles G	14 ml
Carrier RNA	310 μg
Proteinase K	180 mg
Protease Dissolve Buffer	10 ml
Buffer MLK	250 ml
Buffer MAW1	250 ml
Buffer MW2*	2 x 50 ml
Elution Buffer	60 ml

Contents	IVD5432-F-96A	IVD5432-F-96B	IVD5432-F-96C
Sample amount	200～350μl	400~700μl
Carrier RNA	110 μg	110 μg
Proteinase K	50 mg	100 mg
Protease Dissolve Buffer	5 ml	6 ml
Elution Buffer	15 ml	15 ml
Tip	1	1
Sample Plate A	500μl Buffer MLK	500μl Buffer MLK
Sample Plate B	/	500μl Buffer MLK
Wash Plate 1	700μl Buffer MAW1	700μl Buffer MAW1
Wash Plate 2	25μl Buffer MPG2700μl Buffer MW2	25μl Buffer MPG2700μl Buffer MW2
Wash Plate 3	700μl Buffer MW2	700μl Buffer MW2
Elution Plate	/	/

Contents	IVD5432-TL-06A	IVD5432-TL-06B	IVD5432-TL-06C
Sample amount	300~350μl	600~700μl	900~1050μL
Carrier RNA	310 μg	310 μg	310 μg
Proteinase K	50 mg	100 mg	150 mg
Protease Dissolve Buffer	6 ml	6 ml	10 ml
Elution Buffer	15 ml	15 ml	15 ml
DA-Tip	12	12	12
Row 1/7	600μl Buffer MLK	600μl Buffer MLK	600μl Buffer MLK
Row 2/8	/	600μl Buffer MLK	600μl Buffer MLK
Row 3/9	600μl Buffer MAW1	600μl Buffer MAW1	600μl Buffer MLK
Row 4/10	20μl Buffer MPG2600μl Buffer MW2	20μl Buffer MPG2600μl Buffer MW2	30μl Buffer MPG2900μl Buffer MAW1
Row 5/11	600μl Buffer MW2	600μl Buffer MW2	900μl Buffer MW2
Row 6/12	/	/	/

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

MagPure Circulating DNA Mini Kit is designed for purification of high quality circulating DNA (cfDNA) fromcell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Overview

Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.

• Heat sealing is a quick and cost effective method of plate sealing
• Our Gas PermASeal II Heat Seal is a paper with a grid lacquer coating which is porous and gas permeable
• The Gas PermeASeal II Heat Seal is compatible with polypropylene, polyethylene, polystyrene and cyclic olefin copolymer (COC) plates
• The seal can be removed by peeling, or it can be pierced with a pipette tip manually or using a liquid handling robot
• The Seal has a temperature range from -20°C to 80°C
• It can be used for insect and seed storage, as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in roll format compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Peelable heat sealing film which is made using gas permeable grid lacquer paper. This seal is suitable for insect studies and seed storage.