N-(alcohol-PEG2)-N-bis(PEG2-propargyl) has a hydroxyl group and two propargyl groups. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. The propargyl groups can participate in copper catalyzed azide-alkyne Click Chemistry.
N-(alcohol-PEG2)-N-bis(PEG2-propargyl) has a hydroxyl group and two propargyl groups. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. The propargyl groups can participate in copper catalyzed azide-alkyne Click Chemistry.
Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TaqMan 2x PCR Master Mix – Cat. 28340
Norgen’s TaqMan 2x PCR Master Mix is a ready-to-use TaqMan 2x PCR Master Mix solution that contains a PCR internal control which can be detected by HEX/VIC channel in a real-time PCR machine. By detecting the internal control users can validate the DNA template quality, thereby preventing any false negatives in the PCR results. The user needs only to add template, target TaqMan primer/probe mix and water to set up the TaqMan real-time PCR.
PCR Control:
TaqMan 2x PCR Master Mix contains PCR control primers/probe (HEX/VIC) and PCR control template. The PCR control reaction in the TaqMan 2x PCR Master Mix is optimized to not interfere with target amplification. The fluorescence of the target probe should not be HEX/VIC.
Reagents Supplied – Cat # 28340
Storage Conditions and Product Stability
Norgen’s TaqMan 2x PCR and 2x RT-PCR Master Mixes should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles. When stored at the proper temperature this reagent is stable for at least 1 year.
HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Purification and concentration of RNA（mRNA>200nt or miRNA）from transcription products, DNase cleavage products, labeled products, and crude RNA products |
| Applications | Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Crude RNA, enzymatic reaction solution |
| Sample amount | ≤100μl |
| Recovery | 90% |
| Elution volume | ≥15μl |
| Time per run | ≤15 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Advantages
Kit Contents
| Contents | R214402 | R214403 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer GXP | 30 ml | 120 ml |
| Buffer RW2 | 20 ml | 2 x 50 ml |
| RNase-Free Water | 20 ml | 60 ml |
| HiPure RNA Mini Columns I | 50 | 250 |
| 2 ml Collection Tubes | 50 | 250 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.
