N-(Amine-PEG8)-N-bis(PEG8-Propargyl)

Designed for those labs wishing to separate magnetic beads into rings.

The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies. 

SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot 

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

RLE500R

Features

• SBS/ SLAS footprint
• Integrated cushion base for precise pipetting
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated bracket to help keep microplate in place and flat
• Made in USA 
• For Research use only

Compatibility

• Full- skirt PCR plates
• 1/2- skirt PCR plates 
• Un-skirted PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. SPTLabtech Firefly®, Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 150 µL 

Minimum  – 5 µL 

Designed for those labs wishing to separate magnetic beads into rings.

The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.

Description 

The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.

Spectral Characteristics

When it is bound with bovine serum albumin (BSA), the fluorescent emission of FluoroStain Protein Fluorescent Staining Dye can be excited by UV and blue light sources, with excitation peaks around 369 and 517 nm and emission at 605 nm. In absence of BSA, FluoroStain Protein Fluorescent Staining Dye shows ignorable fluorescence as compared with protein-bound form, therefore giving a clear background for photographic analysis.

These spectral characteristics made this fluorescent dye compatible with a wide variety of gel reading facilities, including UV/ blue light epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners.

Storage 

Protected from light 
-20°C for 24 months 

The FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain, the FluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option rather than a requirement. With further reduction of background signals via destaining process, the FluoroStain™ is capable of achieving detection level parallel to silver staining without specialized imaging equipment, making it one of the most sensitive dyes available. In addition to its remarkable sensitivity, the FluoroStain™ Protein Fluorescent Staining Dye (Red, 1000×) brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding the risk of skin/ eye damage caused by UV light. For best result, we suggest using B-BOX™ Blue Light LED epi-illuminator to visualize and analyze the gel stained with FluoroStain Protein Fluorescent Staining Dye (Red, 1000×). The FluoroStain™ Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra, i.e. LC-MS/MS, MALDI-TOF, etc.

Overview

• Fast and easy processing using either a rapid spin column or automation friendly high-throughput 96-well plate format
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• Bind & elute all RNA irrespective of size or GC content, without bias
• Highly sensitive & linear down to a few cells/viral copies without the need for carrier RNA
• No phenol or chloroform extractions
• For use on preserved saliva collected with Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200)
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• CE-IVD marked version available

Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.

Saliva/Swab RNA Purification Kit (Spin Column)

This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.

Saliva/Swab RNA Purification 96-Well Kit (High Throughput)

High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.

Details

Supporting Data

Figure 1 / 8

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Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.