N-(Amine-PEG8)-N-bis(PEG8-Propargyl) is a trifunctional linker containing an amino group and two terminal alkynes. These alkynes are frequently used in copper (I) click reactions with azides to form stable triazole linkages, while the primary amine is reactive towards carboxylic acids and other carbonyl compounds such as NHS esters, aldehydes or ketones.
N-(Amine-PEG8)-N-bis(PEG8-Propargyl) is a trifunctional linker containing an amino group and two terminal alkynes. These alkynes are frequently used in copper (I) click reactions with azides to form stable triazole linkages, while the primary amine is reactive towards carboxylic acids and other carbonyl compounds such as NHS esters, aldehydes or ketones.
Propargyl-PEG24-amine is a linker with 24 PEG units. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds under the catalyzation of copper. This linker has pretty good water-solubility due to its long PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG24-amine is a linker with 24 PEG units. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group is reactive with azide compounds under the catalyzation of copper. This linker has pretty good water-solubility due to its long PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Intended Use
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
| Ingredients | /liter |
| Tryptose | 20.0g |
| Yeast extract | 5.0g |
| Glucose | 2.0g |
| Disodium hydrogen phosphate | 4.0g |
| Sodium azide | 0.4g |
| 2,3,5-Triphenyl Tetrazolium chloride | 0.1g |
| Agar | 10.0g |
| pH 7.2±0.1 at 25°C | |
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
| Quality control strains | Growth | Colony color |
| Enterococcus faecalis ATCC29212 | Good growth,PR≥0.5 | deep red coloured colonies |
| Escherichia coli ATCC25922 | Total inhibition | – |
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
