N-(Amine-PEG8)-N-bis(PEG8-Propargyl)

Description

• Description

Aflatoxin is the most common food toxin that is harmful to human and animal health. The most frequent aflatoxins are B1, B2, G1, and G2, which can affect the body through respiratory, mucosal, or cutaneous routes, causing an excessive inflammatory response. Aflatoxin can infect crops during their growing stages or even after they are harvested. It mainly targets the liver and can impair the effectiveness of immunization in children, increasing the risk of infection. Aflatoxin detection and quantification in food and feed is a critical part of food and feed safety concerns.

Aflatoxin is the most common food toxin that is harmful to human and animal health. The most frequent aflatoxins are B1, B2, G1, and G2, which can affect the body through respiratory, mucosal, or cutaneous routes, causing an excessive inflammatory response. Aflatoxin can infect crops during their growing stages or even after they are harvested. It mainly targets the liver and can impair the effectiveness of immunization in children, increasing the risk of infection. Aflatoxin detection and quantification in food and feed is a critical part of food and feed safety concerns.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

ChIP-Seq Library Prep Kit Workflow

ChIP-Seq is the combination of chromatin immunoprecipitation (ChIP) with next generation sequencing. It is a powerful tool for the analysis of global transcription factors and other proteins in diseases and biological pathways, and characterization of histone modifications in a genome-wide level at single-base resolution. ChIP-Seq delivers whole genome level of functional profiling of global transcription factors, and provides better understanding of epigenetic modifications.

Three index types are available for the ChIP-Seq Library Prep Kit of the illumina platform:

Non-index (Cat.# 30032): Libraries do not have index.

Index (Cat.# 30034): Each index primer contains a unique 6-base index sequence can be used for identification. 48 samples can be pooled together. Index information can be downloaded here.

Unique dual index (Cat.# 30036): The ChIP-Seq library multiplexing for 96 samples is possible. Our unique 4-Base Difference Index System have 8 bases index length and at least 4 bases are different from each other for better library identification. Our unique dual indexing primers remove sequencing errors such as index hopping, index contamination, mis-assignment, and other errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34034).

Kit advantages:

• Super fast protocol
  • Library prep can be done in 1.5 hours
  • The hands-on time is only around 10 minutes
• Easy procedure
  • Ready-to-use master mix simplified the procedure
  • Less reaction components make it easy to setup reactions
• Reduced more than half of the beads cost
• Input ChIP DNA: From 5 ng to 400 ng

Comparison of library conversion efficiency under the same condition. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Comparison of aligned reads, aligned rate and duplication rate. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Data comparison: Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used. Sequencing peak regions are shown.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

K-ASPTM

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

Content:	50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Aspartame
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Decrease
Linear Range:	10 to 150 µg of aspartame per assay
Limit of Detection:	0.57 mg/L
Reaction Time (min):	~ 5 min
Application examples:	Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials.
Method recognition:	Novel method

This product has been discontinued (read more).

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

We have a wide range of other assay kit products.

Advantages

• Very cost effective 
• All reagents stable for > 12 months after preparation 
• Only enzymatic kit available 
• Measures aspartame and breakdown products (L-aspartate and aspartame acid) 
• Very specific 
• Very rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.