N-(Propargyl-PEG2)-PEG3-t-butyl ester is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Propargyl-PEG2)-PEG3-t-butyl ester is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Principle

The Kit isolates total RNA from up to 10⁷ cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.

Advantages

• Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – several samples can be extracted in 25 minutes by column method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG

Kit Contents

Storage and Stability

HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.

This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Name of Product

Lactobacillus/Pediococcus-Screen

Catalog Number

MGScLP

Short Info

This test discovers beer germs in a timely and precise manner

Method/Platform

PCR

Range/Assay Sensivity

10^4 – 10^5 cfu/mL

Test Principle

The technological basis for the GenLine tests  is the polymerase chain reaction (PCR) combined with lateral flow tests.

Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.

The resulting PCR products carry the labels of the incorporated primers.

In a second part of the test, the created PCR products are detected by a lateral flow Test Strip.  A “molecular sandwich” is formed and becomes visible as a line on the test Strip.

Brief Instructions
The PCR reagents and the samples are prepared.
After the addition of the sample to the PCR reagents, the PCR is started.
The resulting PCR products are detected by a simple lateral flow test

Storage
-15 to -20°C

Components
PCR-Polymerase Master-Mix, PCR-Primermix, Positive Kit-Control, PCR-Water