Description 

1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system. 

Features

• Reliable: Rigorous quality control for reproducible separation of protein electrophoresis.

• Convenient: Premeasured pouches make 1 liter of 1X buffer solution; No pH adjustment is necessary. 

• Fast: Dissolving in minutes and then ready to use.

• Stable: Powder packaging suitable for long-term storage.

Contents 

50mM Tris Base, 50mM MOPS, 0.1% SDS, 1mM EDTA

Applications 

Running buffer for sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) in Bis-Tris buffer system

Storage

Room temperature for 24 months 

1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system.

Introduction

The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample)
Applications	SDS-PAGE electrophoresis and western blot, etc
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples
Sample amount	Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7

Principle

The Kit isolates total RNA from up to 10 ⁷ cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.

Advantages

• High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
• Fast – column method can complete the extraction of several samples in 30 minutes
• Safe – no phenol chloroform extraction required
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

Kit Contents

Contents	R521102	R521103
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Columns	50	250
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	100	2 x 250
Buffer RLC	50 ml	200 ml
Buffer GW1*	22 ml	66 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	50 ml	3 x 50  ml
RNase Free Water	10 ml	30 ml
Elution Buffer	10 ml	30 ml
Buffer ALO(5%SDS)	10 ml	30 ml

Storage and Stability

HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.

The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Introduction

Usages:
For cultivation of Escherichia coli.

Principle:
Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.

Formulation（per liter）:

Pancreatic Digest of Gelatin	20 g
Lactose Monohydrate	10g
Dehydrated Ox Bile	5g
Bromocresol Purple	10mg
final pH	7.3±0.2

How to use：
1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle 

250g