N-Boc-N-bis(PEG2-propargyl)

K-LOLAC

SKU: 700004314

65 assays per kit

Content:	65 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Lactose
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)
Limit of Detection:	1.62 mg/L
Reaction Time (min):	~ 10 min
Method recognition:	AOAC Method 2020.08

The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.

We offer more monosaccharide and oligosaccharide assay kit products.

View Lactose Assay Kit (K-LOLAC) Flyer

View Product Poster Presentation – Single Lab Validation for K-LOLAC.

Validation of Methods

Advantages

• World’s first sequential assay for lactose, i.e. improves accuracy and efficiency 
• Contains a specific β-galactosidase for the selective measurement of lactose in dairy products 
• Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products 
• Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1.62 mg/L 
• Very competitive price (cost per test)

Introduction

Usages:
For isolating , cultivating mold and yeast .

Principle:
Potato flour leaching contribute various mold growth; glucose to provide energy; agar as medium coagulant; chloramphenicol inhibit the growth of bacteria.

Formulation(per liter):
Infusion from potatoes 200g
Glucose 20g
Agar 15g
Final pH 5.6 ± 0.2

How to use:
1.Suspend 40g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

About

A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.

PACE Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE compatible genotyping assays are comprised of two competitive allele specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer.  PACE Genotyping Master Mix is supplied at 2x concentration and with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.

Genotyping assay designs are available from 3CR Bioscience through our free PACE assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• Template DNA
• PCR-grade water
• Genotyping assays

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

Template DNA

PCR-grade water

Genotyping assays