N-(Boc-PEG3)-N-bis-(PEG3-Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups and a t-Boc protected aminooxy group. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The protected amine can be deprotected under acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Detail
N-(Boc-PEG3)-N-bis-(PEG3-Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups and a t-Boc protected aminooxy group. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The protected amine can be deprotected under acidic conditions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Other Products
R6611 MagPure Blood RNA Kit
Product Info
Document
Product Info
Introduction
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount
1-1.5ml whole blood, 0.5-1ml bone marrow
Yield
1-30μg
Principle
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.
Advantages
High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
Economy – less than 50% of the price of top brands
Automatic – extraction without manual participation, saving time and effort
High yield – nucleic acid recovery up to 95%
General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.
Kit Contents
Contents
R661101
R661102
R661103
Purification Times
48 Preps
96 Preps
480 preps
10 x RBC Lysis Buffer
50 ml
2 x 50 ml
4 x 100 ml
Proteinase K
12 mg
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
1.8 ml
10 ml
DNase I
600 μl
2 x 600 μl
10 x 600 µl
DNase Buffer
20 ml
30 ml
150 ml
MagPure Particles N
1.2 ml
2.5 ml
11 ml
Buffer RTL
30 ml
60 ml
300 ml
Buffer ALB2
40 ml
60 ml
300 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.
Document
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
t-Boc-aminooxy-PEG5-propargyl is a click crosslinker. Propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. T-Boc-aminooxy can be converted to free aminooxy under mild acidic conditions and then can react with an aldehyde or ketone. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
t-Boc-aminooxy-PEG5-propargyl is a click crosslinker. Propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. T-Boc-aminooxy can be converted to free aminooxy under mild acidic conditions and then can react with an aldehyde or ketone. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.