Description

The EzRNA™ T7 High Yield RNA Synthesis Kit is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.

Features

• High yield
• Versatile- suitable for short and long transcripts
• NTP premixed- Minimal pipetting and setup time
• Compatible with CleanCap® Reagent AG
• Lithium chloride included for RNA purification

Application

• Generation of RNA from T7 promoter-driven DNA sequences
• Suitable for subsequent cap-0 and cap-1 modification

Storage

-20°C for 12 months

The EzRNA™ T7 High Yield RNA Synthesis Kit is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.

Overview

• Extract high quality & quantity total RNA including miRNA 
• Isolate from a wide variety of animal tissues 
• No phenol step required – isolate all RNA in one fraction
• Compatible with tissues stored in RNAlater® or Trizol®  
• Convenient & fast spin column format
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc.  Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

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Storage Conditions and Product Stability
The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.

Introduction

This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit onthe amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.

Details

Principles

SolPure DNA Kits is an improved salt precipitation purification method. (Blood samples are lysed in red blood cell lysis buffer to remove red blood cells, and white blood cells are collected by centrifugation.) After lysis, DNA is released into the lysis buffer. RNA is removed by RNASE A. High salt solution is added to precipitate proteins and impurities. Centrifuge to remove precipitate and obtain supernatant containing only DNA. Add isopropanol to precipitate and recover DNA. Wash with 70% ethanol to remove salt, and finally add Buffer TE to dissolve DNA.

Kit Contents

Storage and Stability

RNase A and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit onthe amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.