N-Me-N-bis(PEG2-propargyl) has two alkyne groups which can participate in Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is required for catalyzation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-Me-N-bis(PEG2-propargyl) has two alkyne groups which can participate in Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is required for catalyzation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-AVCHO
SKU: 700004267
100 Assays of each per kit
| Content: | 100 assays of each per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Available Carbohydrates, Dietary Fiber |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay |
| Limit of Detection: | 1.475 g/100 g |
| Reaction Time (min): | ~ 5 h |
| Application examples: | Food ingredients, food products and other materials. |
| Method recognition: | AOAC Method 2020.07 |
The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving ‘First Action’ status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).
* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.
See our full range of dietary fiber assay kits.
The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving ‘First Action’ status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).
Usages:
For monitoring and detection of surface of equipment and personnel hygiene.
Advantage:
1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life.
2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability.
3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation.
4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit..
5.This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.
Specifications: 55mm*10 plates / bag
55mm*10 plates / bag
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Figure 1 / 5
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| Kit Specifications | |
| Column Binding Capacity | 1.5 mg |
| Average Yield from 100 mL Culture* | 0.4 – 1.0 mg |
| Final Endotoxin Levels | < 0.1 EU/µg DNA |
| Time to Complete 4 Purifications | 1.5 hours |
| Size of Plasmids Purified | Up to 13,000 bp |
* Depends on high-copy DNA and low copy DNA
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.
| Component | Cat. 13300 (50 preps) | Cat. 46400 (250 preps) | Cat. 46500 (4 preps) | Cat. 46600 (20 preps) | Cat. 60300 (50 preps) | Cat. 63000 (192 preps) |
|---|---|---|---|---|---|---|
| Resuspension Solution AZ | 12 mL | 60 mL | 20 mL | 100 mL | 12 mL | 2 x 20 mL |
| Lysis Buffer N | 40 mL | 80 mL | 40 mL | 2 x 80 mL | 40 mL | 2 x 40 mL |
| Buffer TN | 20 mL | 130 mL | 55 mL | 2 x 130 mL | 20 mL | 1 x 55 mL 1 x 20 mL |
| Wash Solution E | 12 mL | 2 x 18 mL | – | – | – | – |
| Elution Buffer K | 8 mL | 30 mL | – | – | 8 mL | 2 x 8 mL |
| Wash Solution J | – | – | 25 mL | 3 x 25 mL | – | – |
| Elution Buffer J | – | – | 24 mL | 120 mL | – | – |
| RNase A | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | – | – | – | – | 1 x 1.1 mL | 4 x 1.1 mL |
| Spin Columns | 50 | 250 | – | – | – | – |
| Collection Tubes | 50 | 250 | – | – | – | – |
| DNA Maxi Spin Columns with Collection Tubes (Clear ring in column) | – | – | 4 | 20 | – | – |
| Maxi Spin Filter Columns with Collection Tubes (Grey ring in column) | – | – | 4 | 20 | – | – |
| 96-Well Plate | – | – | – | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – | – | 50 | – |
| Elution Tubes (50 mL) | – | – | 4 | 20 | ||
| 96-Well Elution Plate | – | – | – | – | – | 2 |
| Adhesive Tape | – | – | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 |
