N-Me-N-bis(PEG2-propargyl)

Name of Product

Aspergillus Antigen
(Galactomannan) – ELISA

Catalog Number

ASPE-096

Short Info

This ELISA kit is for the quantitative detection of specific Aspergillus
Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).

This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format, TMB, λ=450 nm

Range/Assay Sensivity

Limit of Detection (LOD): 0,5 ng/ml
Limit of Quantification (LOQ): 1,0 ng/ml

Test Principle

FungaDia Aspergillus Galactomannan ELISA Detection Kit is a one-step enzyme sandwich microplate immunoassay which
detects galactomannan in human serum and BAL fluid.
Galactomannan antigen in the sample bind to anti-galactomannan antibodies (sensitized on the microtiter plates) and conjugate (monoclonal antibody/peroxidase). The presence of Galactomannan Antigen is detected with substrate TMB.

12 x 8 microwell plate (96 tests)

This ELISA kit is for the quantitative detection of specific Aspergillus
Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).

This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Product Description

Amp-future DNA Isothermal Amplification Kit NFO: Freeze-Dried Reagent

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Tetra-(amido-PEG10-propargyl) is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.

Tetra-(amido-PEG10-propargyl) is a branched crosslinker with four terminal propargyl groups. The propargyl groups can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage.